THE EFFECT OF SOME GENERAL ANAESTHETICS ON THE SURFACE POTENTIAL OF LIPID MONOLAYERS
THE EFFECT OF SOME GENERAL ANAESTHETICS ON THE SURFACE POTENTIAL OF LIPID MONOLAYERS
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一些普通麻醉剂对脂质单层表面电位的影响
DOI：
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发表时间：
1979
影响因子：
7.3
通讯作者：
W. Mason
中科院分区：
文献类型：
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作者：
A. Bangham;W. Mason
1本研究旨在研究Ginsberg（1978）的报告，即0.7 m乙醇使KCl上形成的单油酸甘油酯（GMO）单层的表面电位发生+100 mV变化（ΔΔV），尽管他预测应该已经发现了−10 mV的ΔΔV。2全身麻醉剂如正烷基醇和戊巴比妥对表面电位（ΔV）的影响使用Americium-241空气电极组件（ΔV）和镀铂铂浸渍板和力天平（γ）检查了由甘油单油酸酯（GMO）或磷脂酰胆碱（PC）在145 mm KCl上形成的脂质单层的表面张力（γ）。3发现，正如Ginsberg（1978）所预测的，向浸泡PC或GMO单层的水相中加入0.7 m乙醇会导致界面电位（ΔΔV）发生负向变化。4 ΔΔV的大小与乙醇浓度呈线性关系。5更长链长的醇直到正癸醇也会引起ΔΔV的负向变化，并且ΔΔV对麻醉剂活性的依赖性，相对于增加麻醉剂的链长，与Traube定律一致。6将戊巴比妥加入到水相中，浸泡单层膜，也会产生负ΔΔV，这一发现排除了挥发性醇吸附到测量电极上的可能性。[7]根据固定偶极子阵列中的无序增加（例如暴露于麻醉剂的双层中可能发生的无序增加）将导致对主要不可渗透阳离子的静电势垒降低的命题，对研究结果进行了讨论。
1 This study sought to investigate the report by Ginsberg (1978) that 0.7 m ethanol brought about a +100 mV change (ΔΔV) in the surface potential of glyceryl monooleate (GMO) monolayers formed on KCl, although he predicted that a ΔΔV of −10 mV should have been found. 2 The effect of general anaesthetics such as n‐alkyl alcohols and pentobarbitone on surface potential (ΔV) and surface tension (γ) of lipid monolayers formed on 145 mm KCl from either glyceryl monooleate (GMO) or phosphatidyl choline (PC) was examined with an Americium‐241 air electrode assembly (ΔV) and a platinized platinum dipping plate and force balance (γ). 3 It was found that, as predicted by Ginsberg (1978), addition of 0.7 m ethanol to the aqueous phase bathing either PC or GMO monolayers brings about a negative‐going change in interfacial potential (ΔΔV). 4 The magnitude of ΔΔV is dependent in a linear fashion on ethanol concentration. 5 Longer chain length alcohols up to n‐decanol also bring about a negative going change in ΔΔV, and the dependence of ΔΔV on anaesthetic activity, with respect to increasing chain length of anaesthetic, is consistent with Traube's law. 6 Pentobarbitone added to the aqueous phase bathing the monolayer also elicits a negative ΔΔV, a finding which rules out the possibility of adsorption of the volatile alcohols to the measuring electrode. 7 The findings are discussed in terms of the proposition that increasing disorder in an array of fixed dipoles, such as might occur in a bilayer exposed to anaesthetic, would result in a lowering of the electrostatic barrier to the predominantly impermeable cation.