Enzyme synthesis following conjugation and recombination in Escherichia coli.
Enzyme synthesis following conjugation and recombination in Escherichia coli.
复制标题
大肠杆菌中缀合和重组后的酶合成。
DOI：
10.1016/0022-2836(68)90004-1
复制
发表时间：
1968
影响因子：
5.6
通讯作者：
O. Siddiqi
中科院分区：
文献类型：
--
作者：
G. Joshi;O. Siddiqi
碱性磷酸酶的结构基因，当注射到一个组成型受体的HFR，不广泛的功能。在最初的爆发之后，磷酸酶的合成受到抑制，直到P+基因整合到受体的染色体中才恢复。遗传重组的时间过程进行了研究，通过以下的合成碱性磷酸酶的等位基因磷酸酶阴性突变体之间的杂交。在这种杂交中出现PP+重组核是由于酶合成的差异速率增加所致。功能性重组体在HfrP基因进入后30至40分钟出现，并从它们产生的时间起呈指数级繁殖。复合的动力学与F−的产生时间无关。在携带在42 °C阻断DNA复制的热敏突变的受体中，重组在DNA复制不存在的情况下发生。事实上，对DNA复制的干扰大大提高了重组的速度。
The structural gene for alkaline phosphatase, when injected by an Hfr into a constitutive recipient, does not function extensively. After an initial spurt, phosphatase synthesis suffers an eclipse and is not resumed until theP+gene is integrated into the recipient's chromosome. The time-course of genetic recombination was investigated by following the synthesis of alkaline phosphatase in crosses between allelic phosphatase-negative mutants. The appearance of PP+recombinant nuclei in such crosses is paralleled by an increase in the differential rate of enzyme synthesis. Functional recombinants arise 30 to 40 minutes after the entry of the HfrPgene and multiply exponentially from the time they originate. The kinetics of recombination are unrelated to the generation time of the F−. In a recipient carrying a thermosensitive mutation which blocks DNA replication at 42 °C, recombination occurs in the absence of DNA replication. Interference with DNA replication, in fact, greatly enhances the rate of recombination.