Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis
Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis
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人 ESCRT 和 ALIX 蛋白与中体蛋白相互作用并在胞质分裂中发挥作用
DOI：
10.1038/emboj.2012.184
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
W. Sundquist
中科院分区：
文献类型：
--
作者：
E. Morita;V. Sandrin;Hyo‐Young Chung;S. Morham;S. Gygi;C. Rodesch;W. Sundquist
我们希望报告说，我们发现图 1D 所示实验中使用的 AD-ROCK1 酵母两种杂交构建体存在错误。该错误在 ROCK1 氨基酸 123 之后引入了一个终止密码子，因此我们报道的 TSG101 和全长 ROCK1 之间的两种杂交相互作用实际上是在 TSG101 和仅表达 ROCK1 前 123 个残基的截短构建体之间。此错误不会影响原始论文图 1B 中显示的相应 ROCK1-TSG101 免疫共沉淀实验。基于免疫共沉淀实验，ROCK1可以与TSG101相互作用的结论成立。
We wish to report that we have discovered an error in the AD-ROCK1 yeast two hybrid construct used in the experiment displayed in Figure 1D. This error introduced a stop codon after ROCK1 amino acid 123, so that the two hybrid interactions that we reported as being between TSG101 and full-length ROCK1 were actually between TSG101 and a truncated construct that expressed only the first 123 residues of ROCK1. This error does not affect the corresponding ROCK1-TSG101 co-immunoprecipitation experiment displayed in Figure 1B of the original paper. Based on the co-immunoprecipitation experiment, the conclusion that ROCK1 can interact with TSG101 stands.