Genetic characterization of Babesia and Theileria parasites in water buffaloes in Sri Lanka.
Genetic characterization of Babesia and Theileria parasites in water buffaloes in Sri Lanka.
复制标题
斯里兰卡水牛巴贝虫和泰勒虫寄生虫的遗传特征。
DOI：
10.1016/j.vetpar.2013.11.029
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Yokoyama N.
中科院分区：
文献类型：
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作者：
Sivakumar T;Tattiyapong M;Fukushi S;Hayashida K;Kothalawala H;Silva SS;Vimalakumar SC;Kanagaratnam R;Meewewa AS;Suthaharan K;Puvirajan T;de Silva WK;Igarashi I;Yokoyama N.
水牛被认为是几种感染牛的血原虫寄生虫的储存宿主。在本研究中，我们调查了斯里兰卡养殖水牛感染巴贝斯虫，二联巴贝斯虫，环形泰勒虫，东方泰勒虫使用寄生虫特异性PCR检测。对斯里兰卡3个不同地区（Polonnaruwa、Mannar和Mullaitivu）饲养的320头水牛的血液DNA样品进行PCR筛选，结果显示B. bovis，牛B. bigemina和T.检测到东方。WhileT.东方蜱为主要寄生虫（82.5%），B. bovis（1.9%）和B.二联胎（1.6%）。本研究扩增了家蚕棒状体相关蛋白1（RAP-1）基因序列。牛B. bigemina和T. orientaliswere比较与以前在斯里兰卡牛的特点。而B。水牛的bigeminaAMA-1序列与牛的bigeminaAMA-1序列具有较高的同源性，B。来自水牛的bovisRAP-1序列在遗传上与牛的那些不同。堡orientalis的MPSP序列，在水牛中未检测到先前报道的斯里兰卡牛MPSP序列类型（1、3、5和7型），本研究分析的MPSP序列属于N1或N2型。总之，除了报告斯里兰卡水牛中巴贝斯虫和泰勒虫的首次基于PCR的调查外，本研究发现水牛源性巴贝斯虫和泰勒虫的主要变体为dB。BovisRAP-1和T. orientalisMPSP序列与以前在这个国家描述的牛的MPSP序列不同。
Water buffaloes are thought to be the reservoir hosts for several hemoprotozoan parasites that infect cattle. In the present study, we surveyed Sri Lankan bred water buffaloes for infections withBabesiabovis,Babesia bigemina, Theileria annulata, andTheileria orientalisusing parasite-specific PCR assays. When 320 blood-derived DNA samples from water buffaloes reared in three different districts (Polonnaruwa, Mannar, and Mullaitivu) of Sri Lanka were PCR screened,B. bovis,B. bigemina, andT. orientaliswere detected. WhileT. orientaliswas the predominant parasite (82.5%), low PCR-positive rates were observed forB. bovis(1.9%) andB. bigemina(1.6%). Amplicons of the gene sequences of the Rhoptry Associated Protein-1 (RAP-1) ofB. bovis, the Apical Membrane Antigen-1 (AMA-1) ofB. bigemina, and the Major Piroplasm Surface Protein (MPSP) ofT. orientaliswere compared with those characterized previously in Sri Lankan cattle. While theB. bigeminaAMA-1 sequences from water buffaloes shared high identity values with those from cattle,B. bovisRAP-1 sequences from water buffaloes diverged genetically from those of cattle. ForT. orientalis, none of the MPSP sequence types reported previously in Sri Lankan cattle (types 1, 3, 5, and 7) were detected in the water buffaloes, and the MPSP sequences analyzed in the present study belonged to types N1 or N2. In summary, in addition to reporting the first PCR-based survey ofBabesiaandTheileriaparasites in water buffaloes in Sri Lanka, the present study found that the predominant variants of water buffalo-derivedB. bovisRAP-1 andT. orientalisMPSP sequences were different from those previously described from cattle in this country.