Engineered chlorophyll catabolism conferring predator resistance for microalgal biomass production
Engineered chlorophyll catabolism conferring predator resistance for microalgal biomass production
复制标题
工程叶绿素分解代谢赋予微藻生物质生产抗捕食者能力
DOI：
10.1016/j.ymben.2021.03.018
复制
发表时间：
2021
影响因子：
8.4
通讯作者：
Tanaka Tsuyoshi
中科院分区：
文献类型：
--
作者：
Kashiyama Yuichiro;Ishizuka Yuki;Terauchi Issei;Matsuda Toshiki;Maeda Yoshiaki;Yoshino Tomoko;Matsumoto Mitsufumi;Yabuki Akinori;Bowler Chris;Tanaka Tsuyoshi
生产有价值的化合物，包括从微藻中提取的生物燃料和药物前体，引起了人们的极大兴趣。稳定的藻类生物量生产对于微藻产业的商业可行性至关重要。然而，最大的问题之一是捕食者对藻类生物量的严重生物污染，导致户外养殖崩溃。在本研究中，我们提出了一种新的微藻应对捕食者的工程策略。植物叶绿素酶(CLH)在微藻中的过表达导致对捕食者的抗性增强。这一结果支持了我们的假设，即当微藻被捕食者消化时，CLH促进了叶绿体中的叶绿素分解，产生了光毒分解代谢的叶绿素，从而损害了捕食者。据我们所知，这是第一次通过提高CLH活性来建立抗捕食者的微藻的研究。
Production of valuable compounds including biofuels and pharmaceutical precursors derived from microalgae has garnered significant interest. Stable production of algal biomass is essential to make the microalgal industry commercially feasible. However, one of the largest issues is severe biological contamination by predators grazing the algal biomass, resulting in the crash of outdoor cultures. In the present study, we propose a novel engineering strategy for microalgae to cope with predators. The overexpression of plant chlorophyllase (CLH) in a microalga resulted in the enhancement of resistance to the predator. This result supported our hypothesis that CLH promotes chlorophyll breakdown in the chloroplasts of the microalgae when they are digested by the predator, generating the phototoxic catabolite chlorophyllide that damages the predator. To the best of our knowledge, this is the first study to establish predator-resistant microalgae by enhancing the CLH activity.