Echocardiographic speckle-tracking based strain imaging for rapid cardiovascular phenotyping in mice.
Echocardiographic speckle-tracking based strain imaging for rapid cardiovascular phenotyping in mice.
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DOI：
10.1161/circresaha.110.239574
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发表时间：
2011-04-15
影响因子：
20.1
通讯作者：
Liao R
中科院分区：
文献类型：
--
作者：
Bauer M;Cheng S;Jain M;Ngoy S;Theodoropoulos C;Trujillo A;Lin FC;Liao R
心脏功能的高灵敏度体内表型分析对于在心血管疾病的小动物模型中评估感兴趣的基因和新疗法是必不可少的。经胸超声心动图是目前用于评估心脏结构和功能的主要方法;然而，标准超声心动图技术对心脏性能的早期或细微变化相对不敏感，特别是在小鼠中。开发并验证超声心动图应变成像方法，用于小动物模型中敏感和快速的心脏表型分型。在这里，我们描述了一种改进的超声心动图技术，利用斑点跟踪为基础的应变分析的非侵入性评估成年小鼠的心脏性能。该方法被发现是快速的，可重复的，高度敏感的评估区域和全球左心室（LV）功能。与常规超声心动图测量的左心室结构和功能相比，峰值纵向应变和应变率能够在心肌梗死后（MI后）的较早时间点检测成年小鼠心脏的变化，并预测不良左心室重构的后期发展。此外，基于斑点追踪的应变分析能够清楚地识别标准MI后心脏治疗早期发生的LV功能的细微改善。我们的研究结果突出了斑点跟踪应变成像的实用性，用于检测心血管疾病小鼠模型中的离散功能改变，以有效和全面的方式。基于超声心动图斑点追踪的应变分析代表了一种相对高通量和灵敏的心脏表型分析方法，特别是在评价小鼠中新兴的心脏药物和治疗方法中。
High-sensitivity in vivo phenotyping of cardiac function is essential for evaluating genes of interest and novel therapies in small animal models of cardiovascular disease. Transthoracic echocardiography is the principal method currently used for assessing cardiac structure and function; however, standard echocardiographic techniques are relatively insensitive to early or subtle changes in cardiac performance, particularly in mice. To develop and validate an echocardiographic strain imaging methodology for sensitive and rapid cardiac phenotyping in small animal models. Herein, we describe a modified echocardiographic technique that utilizes speckle-tracking based strain analysis for the non-invasive evaluation of cardiac performance in adult mice. This method is found to be rapid, reproducible, and highly sensitive in assessing both regional and global left ventricular (LV) function. Compared to conventional echocardiographic measures of LV structure and function, peak longitudinal strain and strain rate were able to detect changes in adult mouse hearts at an earlier time point following myocardial infarction (post-MI) and predicted the later development of adverse LV remodeling. Moreover, speckle-tracking based strain analysis was able to clearly identify subtle improvement in LV function that occurred early in response to standard post-MI cardiac therapy. Our results highlight the utility of speckle-tracking based strain imaging for detecting discrete functional alterations in mouse models of cardiovascular disease in an efficient and comprehensive manner. Echocardiography speckle-tracking based strain analysis represents a method for relatively high-throughput and sensitive cardiac phenotyping, particularly in evaluating emerging cardiac agents and therapies in mice.