Morphological assessment of early axonal regeneration in end-to-side nerve coaptation models
Morphological assessment of early axonal regeneration in end-to-side nerve coaptation models
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DOI：
10.3109/2000656x.2012.696264
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发表时间：
2012-09
影响因子：
1.2
通讯作者：
Hiroshi Oyamatsu;D. Koga;M. Igarashi;M. Shibata;T. Ushiki
中科院分区：
文献类型：
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作者：
Hiroshi Oyamatsu;D. Koga;M. Igarashi;M. Shibata;T. Ushiki
摘要观察端侧神经吻合后周围神经的组织学变化，以确定手术中神经周围神经开放和故意供体神经损伤的影响。将20只大鼠随机分为4组：1组，神经端侧吻合不做神经外膜切开；2组，神经端侧吻合+单纯神经外膜切开；3组，神经端侧吻合+部分挤压伤；4组，神经外膜切开后端侧神经吻合+部分切断。肌皮神经与尺神经吻合7天后，用抗神经丝蛋白-H(RT97)和磷酸化生长相关蛋白-43(p-GAP-43)的抗体进行免疫组织化学分析。前者标记所有轴突，包括再生轴突和变性轴突碎片，而后者仅标记再生轴突。结果显示，第1组受区神经内无再生神经。第2组，由于神经外膜切开部分损伤供体神经纤维，部分再生轴突沿部分损伤的供区神经纤维向近端和远侧延伸，部分再生轴突也通过神经膜切开进入受体神经。与第2组相比，第3组和第4组受体神经和供体神经再生轴突较薄。统计分析显示，神经外膜切开和故意损伤供体神经提高了端侧神经吻合的效果，提示部分神经纤维突出加部分轴突或神经切断对端侧神经吻合中有效的轴突再生具有重要意义。
Abstract Histological changes were observed in peripheral nerves following end-to-side nerve coaptation to determine the effects of perineurial opening and deliberate donor nerve injury during surgery. Twenty rats were randomised into four groups as follows: group 1, end-to-side nerve coaptation without perineurial opening; group 2, end-to-side nerve coaptation with simple perineurial opening; group 3, end-to-side nerve coaptation with partial crush injury after perineurial opening; group 4, end-to-side nerve coaptation with partial neurotomy after perineurial opening. Seven days after coaptation of the musculocutaneous (recipient) nerve to the ulnar (donor) nerve, the nerves were immunohistochemically analysed using antibodies against neurofilament-H (RT97) and phosphorylated GAP-43 (p-GAP-43). The former labels all axons, including regenerating axons and degenerated axonal debris, while the latter only labels regenerating axons. Results demonstrated no regenerating nerves in the recipient nerve of group 1. In group 2, because nerve herniation from the perineurial opening partially injured donor nerve fibres, some regenerating axons extended proximally and distally along the partially injured fibres in the donor nerve; some of these regenerating axons also extended into the recipient nerve via the perineurial opening. In groups 3 and 4, thin regenerating axons were more prominent in recipient and donor nerves compared with group 2. Statistical evaluation revealed increased efficacy of perineurial opening and deliberate donor nerve injury in end-to-side nerve coaptation, suggesting that partial nerve fibre herniation with partial axonotmesis or neurotomesis was important for effective axonal regeneration in end-to-side nerve coaptation.