Nucleotide sequences of Japanese isolates of citrus vein enation virus

Nucleotide sequences of Japanese isolates of citrus vein enation virus
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DOI:
10.1007/s00705-016-3139-6
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发表时间:
2017-03-01
影响因子:
2.7
通讯作者:
Iwanami, Toru
Iwanami, Toru
中科院分区:
医学4区
文献类型:
--
作者:
Nakazono-Nagaoka, Eiko;Fujikawa, Takashi;Iwanami, Toru

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确定了诱导静脉生成的 5 个日本柑橘脉生成病毒 (CVEV) 分离株的基因组序列,并与西班牙分离株 VE-1 的基因组序列进行了比较。所有日本分离株的核苷酸序列长度均为 5,983 nt。日本分离株的基因组 RNA 在正义链中具有 5 个潜在的开放阅读框(ORF 0、ORF 1、ORF 2、ORF 3 和 ORF 5)。日本分离株和西班牙分离株VE-1之间的核苷酸序列同一性范围为98.0%至99.8%。比较 10 个日本分离株和 3 个西班牙分离株的部分氨基酸序列表明,ORF 2 中第 83、104 和 113 位以及 ORF 5 中第 41 位的 4 个氨基酸残基可能是某些日本分离株所特有的。
The genomic sequences of five Japanese isolates of citrus vein enation virus (CVEV) isolates that induce vein enation were determined and compared with that of the Spanish isolate VE-1. The nucleotide sequences of all Japanese isolates were 5,983 nt in length. The genomic RNA of Japanese isolates had five potential open reading frames (ORF 0, ORF 1, ORF 2, ORF 3, and ORF 5) in the positive-sense strand. The nucleotide sequence identity among the Japanese isolates and Spanish isolate VE-1 ranged from 98.0% to 99.8%. Comparison of the partial amino acid sequences of ten Japanese isolates and three Spanish isolates suggested that four amino acid residues, at positions of 83, 104, and 113 in ORF 2 and position 41 in ORF 5, might be unique to some Japanese isolates.