ISOLATION AND FUNCTION OF A CLOSTRIDIUM-PERFRINGENS ENTEROTOXIN FRAGMENT
ISOLATION AND FUNCTION OF A CLOSTRIDIUM-PERFRINGENS ENTEROTOXIN FRAGMENT
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DOI：
10.1128/iai.55.12.2912-2915.1987
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发表时间：
1987-12-01
影响因子：
3.1
通讯作者：
SAKAGUCHI, G
中科院分区：
文献类型：
--
作者：
HORIGUCHI, Y;AKAI, T;SAKAGUCHI, G
用2-硝基-5-硫氰基苯甲酸处理产气荚膜梭菌肠毒素，得到一个片段。2-硝基-5-硫氰基苯甲酸是一种特异性地裂解半胱氨酸残基氨基末端肽键的试剂。该片段经高效液相色谱分离纯化，相对分子质量为15,000。该片段对Vero细胞无细胞毒作用，但竞争性抑制肠毒素诱导的~(51)Cr释放。125I标记片段与Vero细胞的结合力与肠毒素相当。此外，~(125)I标记的片段不能与缺乏肠毒素受体的FL细胞结合。我们推测该片段含有肠毒素的结合区。氨基酸组成分析表明，该片段位于肠毒素的羧基末端。
A fragment was obtained by treating Clostridium perfringens enterotoxin with 2-nitro-5-thiocyanobenzoic acid, a reagent which specifically cleaves the amino-terminal peptide bond of cysteine residues. The fragment (molecular weight, 15,000) was purified by high-performance liquid chromatography. The fragment had no cytotoxic effect on Vero cells but competitively inhibited enterotoxin-induced 51Cr release. Binding of 125I-labeled fragment to Vero cells was comparable to that of enterotoxin. Moverover, 125I-labeled fragment did not bind to FL cells, which lack receptor for enterotoxin. We conclude that the fragment contains the binding domain of enterotoxin. The amino acid composition of the fragment suggests that it is located on the carboxyl-terminal part of enterotoxin.