ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos
ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos
复制标题
斑马鱼：一个灵活的平台，用于分析斑马鱼胚胎的心脏速率
DOI：
10.1038/srep04898
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发表时间：
2014-05-09
影响因子：
4.6
通讯作者：
Santoro MM
中科院分区：
文献类型：
--
作者：
De Luca E;Zaccaria GM;Hadhoud M;Rizzo G;Ponzini R;Morbiducci U;Santoro MM
心跳测量在评估心脏功能方面很重要，因为心律的变化可能是隐藏的病理性心脏状况的原因和影响。斑马鱼（Danio rerio）已成为心脏研究中最有用的模式生物之一。事实上，斑马鱼心脏很容易进行光学分析，无需进行侵入性手术，并且显示出与人类心脏的解剖相似性。在这项研究中，我们提出了一个非侵入性的，简单的，具有成本效益的过程来量化胚胎斑马鱼的心跳。为了实现再现性、高通量和灵活性（即，适应任何现有的共聚焦显微镜系统，并与用户友好的界面，可以很容易地被研究人员使用），我们实现了这种方法在一个软件程序。我们在这里展示了这个名为ZebraBeat的平台，它可以成功地检测胚胎斑马鱼在不同发育阶段的心率变化，并且它可以记录由温度和遗传和化学诱导的改变等因素引起的心率波动。该方法的应用可能包括筛选影响心律的化学文库和从大规模正向遗传筛选中鉴定突变体中的心律变化。
Heartbeat measurement is important in assesssing cardiac function because variations in heart rhythm can be the cause as well as an effect of hidden pathological heart conditions. Zebrafish (Danio rerio) has emerged as one of the most useful model organisms for cardiac research. Indeed, the zebrafish heart is easily accessible for optical analyses without conducting invasive procedures and shows anatomical similarity to the human heart. In this study, we present a non-invasive, simple, cost-effective process to quantify the heartbeat in embryonic zebrafish. To achieve reproducibility, high throughput and flexibility (i.e., adaptability to any existing confocal microscope system and with a user-friendly interface that can be easily used by researchers), we implemented this method within a software program. We show here that this platform, called ZebraBeat, can successfully detect heart rate variations in embryonic zebrafish at various developmental stages, and it can record cardiac rate fluctuations induced by factors such as temperature and genetic- and chemical-induced alterations. Applications of this methodology may include the screening of chemical libraries affecting heart rhythm and the identification of heart rhythm variations in mutants from large-scale forward genetic screens.