Novel methodology for fabrication of tissue-engineered tubular constructs using magnetite nanoparticles and magnetic force
Novel methodology for fabrication of tissue-engineered tubular constructs using magnetite nanoparticles and magnetic force
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DOI：
10.1089/ten.2005.11.1553
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发表时间：
2005-09-01
影响因子：
--
通讯作者：
Honda, H
中科院分区：
文献类型：
--
作者：
Ito, A;Ino, K;Honda, H
创造具有复杂形状的三维结构的新技术将在组织工程中非常有用。在本研究中，利用磁力构建管状结构。阳离子脂质体中的磁铁矿纳米颗粒被靶细胞吸收。将磁性标记的细胞播种到超低附着板上，并在孔下放置磁铁。培养24 h后，磁性标记的细胞形成细胞片。随后，当一个圆柱形磁铁被滚动到电池片上时，电池片被磁铁吸引并在其周围形成一个管。然后将磁铁移除，留下一个管状结构。两种类型的组织被用来创建管状结构：尿组织，由单型尿路上皮细胞层组成；血管组织，由内皮细胞、平滑肌细胞和成纤维细胞的异型层组成。目前的结果表明，这种使用磁铁矿纳米颗粒和磁力的新方法，我们称之为“磁力组织工程”（磁力- te），是一种很有前途的构建组织工程管状结构的方法。
Novel technologies for creating three-dimensional constructs with complex shapes would be highly useful in tissue engineering. In the present study, tubular structures were constructed using magnetic force. Magnetite nanoparticles in cationic liposomes were taken up by target cells. The magnetically labeled cells were seeded onto ultralow-attachment plates, and a magnet was placed under the wells. After 24 h of culture, the magnetically labeled cells formed a cell sheet. Subsequently, when a cylindrical magnet was rolled onto the cell sheet, the cell sheet was attracted to the magnet and formed a tube around it. The magnet was then removed, leaving behind a tubular structure. Two types of tissue were used to create tubular structures: urinary tissue, consisting of a monotypic urothelial cell layer; and vascular tissue, consisting of heterotypic layers of endothelial cells, smooth muscle cells, and fibroblasts. The present results suggest that this novel methodology using magnetite nanoparticles and magnetic force, which we have termed "magnetic force-based tissue engineering" ( Mag-TE), is a promising approach to constructing tissue-engineered tubular structures.