Comparison of the biomechanical tensile and compressive properties of decellularised and natural porcine meniscus
Comparison of the biomechanical tensile and compressive properties of decellularised and natural porcine meniscus
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DOI：
10.1016/j.jbiomech.2015.02.044
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发表时间：
2015-06-01
影响因子：
2.4
通讯作者：
Fisher, J.
中科院分区：
文献类型：
--
作者：
Abdelgaied, A.;Stanley, M.;Fisher, J.
半月板修复被广泛用于半月板损伤的治疗。然而，在半月板损伤已经进展到无法修复的情况下，目前正在开发部分半月板置换的方法，这种方法有可能恢复半月板的功能，在稳定膝关节、吸收和分配负荷期间的应力，并防止早期退行性关节疾病。目前缺乏半月板替代物的一个有吸引力的潜在解决方案是使用脱细胞天然生物支架，这种支架来自异种组织，通过处理天然组织来去除免疫原细胞而产生。本研究通过实验-计算方法研究了脱细胞对猪内侧半月板生物力学拉伸和压缩(凹陷和无约束)特性的影响。结果表明，脱细胞猪内侧半月板保持了天然半月板的拉伸生物力学性能，但具有较低的拉伸初始弹性模量。在压缩状态下，脱细胞猪内侧半月板与天然半月板相比，表现出较低的弹性模量值和较高的渗透率。这些生物力学性质的变化，从不到1%到40%，可能是由于脱细胞过程中糖胺聚糖(GAG)含量的减少。预测的脱细胞猪内侧半月板的生物力学性能在人类半月板的报道范围内，使其成为适合考虑作为部分半月板替代物的生物支架。(C)2015年提交人。爱思唯尔有限公司出版。
Meniscal repair is widely used as a treatment for meniscus injury. However, where meniscal damage has progressed such that repair is not possible, approaches for partial meniscus replacement are now being developed which have the potential to restore the functional role of the meniscus, in stabilising the knee joint, absorbing and distributing stress during loading, and prevent early degenerative joint disease. One attractive potential solution to the current lack of meniscal replacements is the use of decellularised natural biological scaffolds, derived from xenogeneic tissues, which are produced by treating the native tissue to remove the immunogenic cells. The current study investigated the effect of decellularisation on the biomechanical tensile and compressive (indentation and unconfined) properties of the porcine medial meniscus through an experimental-computational approach. The results showed that decellularised medial porcine meniscus maintained the tensile biomechanical properties of the native meniscus, but had lower tensile initial elastic modulus. In compression, decellularised medial porcine meniscus generally showed lower elastic modulus and higher permeability compared to that of the native meniscus. These changes in the biomechanical properties, which ranged from less than 1% to 40%, may be due to the reduction of glycosaminoglycans (GAG) content during the decellularisation process. The predicted biomechanical properties for the decellularised medial porcine meniscus were within the reported range for the human meniscus, making it an appropriate biological scaffold for consideration as a partial meniscus replacement. (C) 2015 The Authors. Published by Elsevier Ltd.