Ultrathin Self-Assembled Diphenylalanine Nanosheets through a Gold-Stabilized Strategy for High-Efficiency Adsorption/Desorption/Ionization
Ultrathin Self-Assembled Diphenylalanine Nanosheets through a Gold-Stabilized Strategy for High-Efficiency Adsorption/Desorption/Ionization
复制标题
通过金稳定策略实现超薄自组装二苯丙氨酸纳米片，实现高效吸附/解吸/电离
DOI：
10.1021/acs.analchem.8b01855
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发表时间：
2018-07-17
影响因子：
7.4
通讯作者：
Ouyang, Gangfeng
中科院分区：
文献类型：
--
作者：
Huang, Siming;Chen, Guosheng;Ouyang, Gangfeng
本文首次采用金稳定策略，采用简单的电喷雾法和热处理工艺制备了超薄、坚固的二苯丙氨酸（FF）自组装纳米片。研究结果表明，金纳米颗粒（AuNPs）与FF纳米片暴露的氨基之间的稳定结合和位阻效应协同作用，导致复合材料具有较强的热稳定性和耐溶剂性。利用FF/Au纳米片优异的表面可达性和较强的激光吸收能力，原位制备了固相微萃取（SPME）光纤和表面辅助激光解吸/电离（SALDI）平台两种强大的功能器件，用于体外和体内生物分析。结果表明，所制备的平台具有两个优点：(1)吸收/解吸速度快（5 min内）；(2)电离效率显著提高2个数量级。结果表明，SPME纤维的提取效率和SALDI平台的定量能力得到了显著提高。该研究不仅证明了FF/Au复合材料可以通过电喷雾+热处理的方法制备，作为具有特定应用前景的吸附/解吸/电离材料，而且为SPME与LDI技术的未来结合提供了有益的策略。
Herein, the ultrathin and robust diphenylalanine (FF) self-assembled nanosheets were fabricated by a gold-stabilized strategy for the first time, using a facile electrospray method followed by a thermal treatment process. The key for the gold-stabilized mechanism was explored, demonstrating that the synergy of the stable binding and steric effect between gold nanoparticles (AuNPs) and the exposed amino groups of FF nanosheets, led to strong thermal stability and solvent resistance of the composites. Contributing to the features of remarkable accessible surfaces and strong laser light absorption ability of this FF/Au nanosheets, two robust functional devices, that is, solid-phase microextraction (SPME) fiber and surface-assisted laser desorption/ionization (SALDI) platforms, were in situ prepared for in vitro and in vivo biological analysis. The findings indicated that the fabricated platforms possessed two advantages: (1) rapid absorption/desorption speed (within 5 min) and (2) remarkable enhancement of ionization efficiency with 2 orders of magnitude. As a result, the extraction efficiency of the SPME fiber and the quantitation ability of SALDI platform were significantly improved. This study not only demonstrated that FF/Au composites could be prepared through an electrospray method followed with thermal-treatment to serve as promising adsorption/desorption/ionization materials for specific applications but also provided useful strategy to advance the ideas for future combination of SPME with LDI technique.