Agaribacterium haliotis gen. nov., sp nov., isolated from abalone faeces
Agaribacterium haliotis gen. nov., sp nov., isolated from abalone faeces
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鲍鱼属
DOI：
10.1099/ijsem.0.002199
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发表时间：
2017
影响因子：
2.8
通讯作者：
Shao Zongze
中科院分区：
文献类型：
--
作者：
Huang Zhaobin;Lai Qiliang;Zhang Demin;Shao Zongze
从鲍鱼的排泄物中分离出一种海洋细菌，命名为“feces2T”菌株。该细菌呈革兰氏染色阴性，呈杆状，具有极鞭毛。它在琼脂平板上形成白色、小、火山口状菌落，具有降解琼脂的能力。氧化酶活性呈阳性，过氧化氢酶活性呈阴性。菌株 feces2T 在 16 至 40°C 下生长，最适温度为 28–30°C。菌株feces2T的近全长16S rRNA基因与MarinibactrumhalimedaeQ-192T的序列相似性最大，为92.9 ％，其次是与TeredinibacterturneraeT7902T的92.8 ％。系统发育分析表明，菌株feces2T属于细胞弧菌科，代表一个独立的进化枝，其未培养的细菌克隆NEP3-15（16S rRNA基因的序列相似性为98 ％）来自浒苔菌藻圈。呼吸醌是泛醌Q-8。主要脂肪酸由总和特征 8 (C18 : 1ω7c/C18: 1ω6c)、C16 : 0 和总和特征 3 (C16 : 1ω6c/C16: 1ω7c) 组成。极性脂质被鉴定为磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未鉴定的氨基磷脂和四种未知脂质。基因组DNA G+C含量为50.5mol％。基于多相特征，菌株feces2T代表了Gammaproteobacteria内细胞振动目细胞振动科中的一个新种和一个新属，其名称为Agaribacterhaliotisgen。十一月，SP。十一月建议菌株为feces2T(=MCCC 1A11450T=KCTC 52708T)。
A marine bacterium, named strain feces2T, was isolated from the excreted faeces of an abalone,Haliotis discus hannai. The bacterium was Gram-stain-negative, rod-shaped and had a polar flagellum. It formed a white, small and crater-like colony on an agar plate, and had the capability of degrading agar. Activity of oxidase was positive and that of catalase was negative. Strain feces2Tgrew at 16 to 40 °C with an optimum of 28–30 °C. The nearly full-length 16S rRNA gene of strain feces2Thad the greatest sequence similarity of 92.9 % withMarinibactrumhalimedaeQ-192T, followed by of 92.8 % withTeredinibacterturneraeT7902T. Phylogenetic analysis indicated that strain feces2Tbelonged to the familyCellvibrionaceae, representing an independent clade with an uncultured bacterium clone NEP3-15 (98 % sequence similarity of 16S rRNA gene) derived from the phycosphere ofEnteromorphaprolifera. The respiratory quinone was ubiquinone Q-8. The predominant fatty acids consisted of summed feature 8 (C18 : 1ω7c/C18: 1ω6c), C16 : 0and summed feature 3 (C16 : 1ω6c/C16: 1ω7c). The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified amino phospholipid and four unknown lipids. The genomic DNA G+C content was 50.5 mol%. On the basis of polyphasic characterizations, strain feces2Trepresented a novel species and a novel genus in the familyCellvibrionaceaeof the orderCellvibrionaleswithin theGammaproteobacteria, for which the nameAgaribacteriumhaliotisgen. nov., sp. nov. is proposed, with the type strain being feces2T(=MCCC 1A11450T=KCTC 52708T).