Using Noninvasive Genetic Sampling to Survey Rare Butterfly Populations
Using Noninvasive Genetic Sampling to Survey Rare Butterfly Populations
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DOI：
10.3390/insects10100311
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发表时间：
2019-10-01
期刊：
影响因子：
3
通讯作者：
Rossetti, Kristin
中科院分区：
文献类型：
--
作者：
Storer, Caroline;Daniels, Jaret;Rossetti, Kristin
非破坏性遗传取样技术的进步继续为研究生物体提供新的机会，特别是那些传统的侵入性取样方法往往无法获得的保护问题。作为概念验证的一部分，我们研究了使用残留的蝴蝶卵碎片的绒毛膜作为可行的遗传物质来源进行分析的有效性。来自联邦濒危的迈阿密蓝蝶（Cyclargus thomasi bethunebakeri）的圈养繁殖种群的实验室材料被用来测试功效和改进方法。随后，使用从现存的北佛罗里达种群的风险霜冻小翅蝶（Callophyrs病毒）现场收集的材料进行评估，由此产生的最佳做法。我们的研究结果表明，它是可能的，以提取足够高的数量和质量的DNA成功的基因测序。我们还描述了一种简单，低成本，可靠的方法，收集和储存鸡蛋碎片样本，可以始终采用现场或实验室工作，以及部署与项目，具有更大的地理范围和/或涉及公民科学家。潜在的限制有关的现场样品收集进行了讨论，以及需要为今后的评价。
Advances in nondestructive genetic sampling techniques continue to offer new opportunities for studying organisms, particularly those of conservation concern where more traditional invasive sampling methods are often not available. As part of a proof-of-concept, we investigated the effectiveness of using the chorion from residual butterfly egg debris as a source of viable genetic material for analysis. Laboratory material from a captive breeding population of the federally endangered Miami blue butterfly (Cyclargus thomasi bethunebakeri) was used to test efficacy and refine the methodology. The resulting best practices were subsequently evaluated using field-collected material from extant north Florida populations of the at-risk frosted elfin butterfly (Callophyrs irus). Our results demonstrated that it is possible to extract DNA of sufficiently high quantity and quality for successful gene sequencing. We additionally describe a simple, low-cost, and reliable method of collecting and storing egg debris samples that can be consistently adopted for field or laboratory work as well as deployed with projects that have a larger geographic scope and/or involve citizen scientists. Potential limitations related to field sample collection are discussed as well as needs for future evaluation.