Intermediate-size filaments: changes in synthesis and distribution in cells of the myogenic and neurogenic lineages.
Intermediate-size filaments: changes in synthesis and distribution in cells of the myogenic and neurogenic lineages.
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中等大小的丝：肌源性和神经源性谱系细胞的合成和分布的变化。
DOI：
10.1101/sqb.1982.046.01.033
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Toyama,Y
中科院分区：
文献类型：
--
作者：
Holtzer,H;Bennett,GS;Tapscott,SJ;Croop,JM;Toyama,Y
中等大小的细丝的数量、它们的地形分布以及与其他细胞器的关系因细胞类型而异。这些10纳米的细丝提供了一个不寻常的细胞器实例，它具有相对恒定的形态，尽管其组成蛋白在分化过程中被改变或取代。本文主要研究了它们在肌发生和神经发生过程中的质和量的变化。我们强调在前体推定成肌细胞和神经母细胞中合成的10纳米纤维类型与它们的子细胞，有丝分裂后成肌细胞和神经母细胞中合成的10纳米纤维类型之间的切换。研究人员讨论了10nm纤维的行为变化对以下因素的响应：(1)阿拉伯糖胞嘧啶（Ara-C），(2)共致癌物12- o -十四烷醇-13-乙酸酯（TPA），(3)与温度敏感的劳斯肉瘤病毒（RSV）的转化，以及(4)细胞密度。我们还报道了秋碱在诱导10环纤维聚集成巨大电缆的作用被紫杉醇（一种微管稳定药物）阻断。
The number of intermediate-size filaments, their topographical distribution, and relationships with other cell organeUes vary among cell types. These 10-nm filaments provide an unusual instance of an organelle that possesses a relatively constant morphology, although its constituent proteins are altered or replaced during differentiation. In this paper we focus on their qualitative and quantitative changes during myogenesis and neurogenesis. We stress the switch between the types of 10-nm filaments synthesized in precursor presumptive myoblasts and neuroblasts and those synthesized in their daughters, the postmitotic myoblasts and neuroblasts. Changes in the behavior of the 10-nm filaments are discussed in response to (1) arabinosylcytosine (Ara-C),(2) the cocarcinogen 12-O-tetradecanoylphorbol-13-acetate (TPA),(3) transformation with temperature-sensitive Rous sarcoma virus (RSV), and (4) cell density. We also report that the effect of Colcemid in inducing aggregation of 10-rim filaments into immense cables is blocked by taxol, a microtubulestabilizing drug.