Physical training modifies the age-related decrease of GAP-43 and synaptophysin in the hippocampal formation in C57BL/6J mouse
Physical training modifies the age-related decrease of GAP-43 and synaptophysin in the hippocampal formation in C57BL/6J mouse
复制标题
DOI：
10.1016/s0006-8993(98)00770-7
复制
发表时间：
1998-09-28
期刊：
影响因子：
2.9
通讯作者：
Wang, SL
中科院分区：
文献类型：
--
作者：
Chen, YC;Chen, QS;Wang, SL
我们研究了从3个月龄开始的适量的长时间体育锻炼对海马结构中GAP-43和突触素表达的影响。将C57BL/6J小鼠分为训练组(24月龄)、安静组(24月龄)和青年组(3月龄)。从3月龄开始，训练组小鼠每天(每周5天)自愿跑轮1h，直到24月龄(21月龄)，而安静组小鼠则放入固定车轮。应用免疫组织化学和图像分析系统对大鼠海马齿状回和CA1、CA3区的GAP-43和突触素进行了定量分析。与幼年小鼠相比，久坐组小鼠海马区GAP-43和突触素免疫染色密度显著降低(P<；0.01)。运动21个月后，训练组小鼠海马区GAP-43和突触素免疫染色的密度较年龄匹配的安静对照组显著增加(P<；0.05，0.01)。这些结果表明，适量的长时间运动训练可以改善大鼠海马区GAP-43和突触素表达的增龄性下降，GAP-43和突触素表达的增加可能与解剖发芽和突触发生有关。(C)1998 Elsevier Science B.V.保留所有权利。
We investigated the effect of a moderate amount of prolonged physical training initiated at 3 months of age on the expression of GAP-43 and synaptophysin in the hippocampal formation. C57BL/6J mice were divided into three groups which were trained (24 months old), sedentary (24 months old) and young (3 months old). From 3 months of age on, mice of trained group were treated with voluntary running wheel for 1 h each day (5 days per week) until 24 months of age (21 months running), whereas mice of sedentary group were put in immobilized wheels for the same time. Using immunohistochemistry and image analysis system, GAP-43 and synaptophysin were analysed quantitatively in the CA1, CA3 areas and the dentate gyrus of the hippocampal formation. As compared with young mice, the densities of GAP-43 and synaptophysin immunostaining showed a significant decrease in the hippocampal formation in sedentary group (P < 0.01). After 21 months of running, the densities of GAP-43 and synaptophysin immunostaining significantly increased in the examined areas of the hippocampal formation in trained mice compared to their age-matched sedentary controls (P < 0.05, 0.01). These results indicate that a moderate amount of prolonged physical training could modify the age-related decrease of the expression of GAP-43 and synaptophysin in the hippocampal formation, and that the increased expression of GAP-43 and synaptophysin might be associated with the anatomical sprouting and synaptogenesis. (C) 1998 Elsevier Science B.V. All rights reserved.