Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons
Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons
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DOI：
10.1117/1.2170579
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发表时间：
2006-01-01
影响因子：
3.5
通讯作者：
Frangioni, JV
中科院分区：
文献类型：
--
作者：
De Grand, AM;Lomnes, SJ;Frangioni, JV
我们演示了如何为近红外 (NIR) 荧光成像应用构建经过校准、稳定且廉价的类组织体模。体模材料由明胶、脂肪乳、血红蛋白和吲哚青绿 (ICG) 组成。吸光度、散射、背景荧光和纹理可以根据需要进行调整。 NIR 荧光内含物由 ICG 标记的聚苯乙烯二乙烯基苯珠和 Pam78 标记的羟基磷灰石晶体组成。前者模拟大小和造影剂浓度可控的肿瘤块，后者模拟乳腺癌中的微钙化。近红外荧光夹杂物可以精确定位在模型中，其中一个或多个区域具有不同的光学特性，并且可以使用微计算机断层扫描独立验证它们的位置。我们演示了如何使用这些模型来校准和比较成像系统，并培训外科医生在近红外荧光图像引导下进行手术。 (c) 2006 年光电仪器工程师协会。
We demonstrate how to construct calibrated, stable, and inexpensive tissue-like phantoms for near-IR (NIR) fluorescence imaging applications. The bulk phantom material is composed of gelatin, intralipid, hemoglobin, and indocyanine green (ICG). Absorbance, scatter, background fluorescence, and texture can be tuned as desired. NIR fluorescent inclusions are comprised of ICG-labeled polystyrene divinylbenzene beads and Pam78-labeled hydroxyapatite crystals. The former mimic tumor masses of controllable size and contrast agent concentration, and the latter mimic microcalcifications in breast cancer. NIR-fluorescent inclusions can be positioned precisely in phantoms, with one or more regions having different optical properties, and their position can be verified independently using microcomputed tomography. We demonstrate how these phantoms can be used to calibrate and compare imaging systems, and to train surgeons to operate under NIR fluorescence image guidance. (c) 2006 Society of Photo-Optical Instrumentation Engineers.