MICROSOMAL-ASSOCIATED GLYCEROPHOSPHATE ACYLTRANSFERASE ACTIVITY IN GERMINATING SOYBEANS
MICROSOMAL-ASSOCIATED GLYCEROPHOSPHATE ACYLTRANSFERASE ACTIVITY IN GERMINATING SOYBEANS
复制标题
DOI：
10.1111/j.1745-4514.1981.tb00672.x
复制
发表时间：
1981-01-01
影响因子：
4
通讯作者：
AMEGAH, R
中科院分区：
文献类型：
--
作者：
CARMAN, GM;HARRINGTON, GF;AMEGAH, R
酰基CoA：sn-甘油-3-磷酸O-酰基转移酶（甘油磷酸酰基转移酶，EC 2.3.1.15）催化甘油-3-磷酸酰化形成溶血磷脂酸。这种酶催化大豆中磷脂和酰基甘油生物合成的第一个关键步骤。甘油磷酸酰基转移酶主要与发芽大豆的微粒体组分相关。反应的最适pH为7.0。该酶对甘油-3-磷酸（Km为0.2 mM）和棕榈酰CoA（Km为6.4 μM）表现出饱和动力学。各种酰基CoA衍生物作为酶的底物。棕榈酰CoA是最有效的酰基CoA底物。添加非离子去污剂Triton X-100可抑制甘油磷酸酰基转移酶活性。硫反应剂抑制酶，表明巯基是必不可少的活动。反应的活化能为8.8千卡每摩尔。微粒体酶在高达70° C的温度下相当稳定。
Acyl CoA:sn‐glycero‐3‐phosphate O‐acyltransferase (glycerophosphate acyltransferase, EC 2.3.1.15) catalyzes the acylation of glycero‐3‐phosphate to form lysophosphatidic acid. This enzyme catalyzes the first committed step in the biosynthesis of phospholipids and acylglycerols in soybeans. Glycerophosphate acyltransferase was predominatly associated with the microsomal fraction of germinating soybeans. The pH optimum for the reaction was 7.0. The enzyme exhibited saturation kinetics for glycero‐3‐phosphate (Kmof 0.2 mM) and palmitoyl CoA (Kmof 6.4 μM). A variety of acyl CoA derivatives served as substrates for the enzyme. Palmitoyl CoA was the most effective acyl CoA substrate. The addition of the nonionic detergent Triton X‐100 inhibited glycerophosphate acyltransferase activity. Thioreactive agents inhibited the enzyme indicating that a sulphydryl group is essential for activity. The activation energy for the reaction was 8.8 Kcal per mole. The microsomal enzyme was reasonably stable to temperatures up to 70° C.