Interleukin 4 induces cultured monocytes/macrophages to form giant multinucleated cells.
Interleukin 4 induces cultured monocytes/macrophages to form giant multinucleated cells.
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DOI：
10.1084/jem.167.2.598
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发表时间：
1988-02-01
期刊：
影响因子：
--
通讯作者：
Rennick DM
中科院分区：
文献类型：
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作者：
McInnes A;Rennick DM
巨型多核细胞（GMC）与肉芽肿性病变相关，肉芽肿性病变是对各种感染性和非感染性病原体的反应而形成的。本研究表明，小鼠 IL-4 通过细胞融合诱导因子依赖性骨髓和肺泡单核细胞在体外形成 GMC。将细胞培养物与 20 U/ml 或更多的 IL-4 一起孵育 2 天后，GMC 出现。抗 IL-4 mAb 阻断这些培养物中 GMC 的出现，表明 IL-4 直接作用于单核细胞以促进融合，并且不会继而诱导其他可溶性融合因子的产生。在软琼脂培养物中，IL-4 还会引起巨噬细胞聚集并减少其迁移。讨论了 IL-4 在肉芽肿炎症反应中的作用。
Giant multinucleated cells (GMCs) are associated with granulomatous lesions that form in response to various infectious and noninfectious agents. The present study shows that mouse IL-4 induces the in vitro formation of GMCs by factor-dependent bone marrow and alveolar monocytes via cell fusion. GMCs appear 2 d after incubation of cell cultures with 20 U/ml or more of IL-4. Anti-IL-4 mAbs block the appearance of GMCs in these cultures, indicating that IL-4 acts directly on monocytes to promote fusion and does not secondarily induce the production of other soluble fusion factors. In soft agar cultures, IL-4 also causes the aggregation of macrophages and diminishes their migration. The role of IL-4 in a granulomatous inflammatory response is discussed.