Hand-held monitor of sympathetic nervous system using salivary amylase activity and its validation by driver fatigue assessment
Hand-held monitor of sympathetic nervous system using salivary amylase activity and its validation by driver fatigue assessment
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DOI：
10.1016/j.bios.2005.03.014
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发表时间：
2006-01-15
影响因子：
12.6
通讯作者：
Mizuno, Y
中科院分区：
文献类型：
--
作者：
Yamaguchi, M;Deguchi, M;Mizuno, Y
为了实现交感神经系统的手持式监测，我们利用干化学系统制作了一个完全自动化的唾液淀粉酶活性分析系统。这是通过制造一种一次性测试条而实现的，该测试条配备有内置的收集和试剂纸以及自动唾液转移装置。为了消除环境温度和唾液pH值变化的影响，通过实验确定了温度和pH值调节方程，并将每个理论值输入手持式监测器的存储器中。在10 - 140 kU/l的唾液淀粉酶活性范围内，手持式监测仪的校准曲线显示系数R-2 = 0.97。因此，证明了该手持式监测器能够使使用者在从收集到完成测量的一分钟内仅用30 μ l唾液样品就以高精度自动测量唾液淀粉酶活性。为了使唾液淀粉酶活性的个体差异在驾驶员疲劳评价中可以忽略不计，提出了一个归一化方程。唾液淀粉酶活性与精神和身体疲劳状态相关。因此，本研究表明，一个很好的手持监测仪与算法的个体的唾液淀粉酶活性的差异，这可以很容易地和快速地用于评估交感神经系统的活动在任何时候的标准化。提示唾液淀粉酶活性可作为一项较好的心理学研究指标。(c)2005 Elsevier B. V.保留所有权利。
In order to realize a hand-held monitor of the sympathetic nervous system, we fabricated a completely automated analytical system for salivary amylase activity using a dry-chemistry system. This was made possible by the fabrication of a disposable test-strip equipped with built-in collecting and reagent papers and an automatic saliva transfer device. In order to cancel out the effects of variations in environmental temperature and pH of saliva, temperature- and pH-adjusted equations were experimentally determined, and each theoretical value was input into the memory of the hand-held monitor. Within a range of salivary amylase activity between 10 and 140 kU/l, the calibration curve for the hand-held monitor showed a coefficient with R-2 = 0.97. Accordingly, it was demonstrated that the hand-held monitor enabled a user to automatically measure the salivary amylase activity with high accuracy with only 30 mu l sample of saliva within a minute from collection to completion of the measurement. In order to make individual variations of salivary amylase activity negligible during driver fatigue assessment, a normalized equation was proposed. The normalized salivary amylase activity correlated with the mental and physical fatigue states. Thus, this study demonstrated that an excellent hand-held monitor with an algorithm for normalization of individuals' differences in salivary amylase activity, which could be easily and quickly used for evaluating the activity of the sympathetic nervous system at any time. Furthermore, it is suggested that the salivary amylase activity might be used as a better index for psychological research. (c) 2005 Elsevier B.V. All rights reserved.