Antibody-decorated dystrophin molecule of murine skeletal myofiber as seen by freeze-etching electron microscopy.
Antibody-decorated dystrophin molecule of murine skeletal myofiber as seen by freeze-etching electron microscopy.
复制标题
通过冷冻蚀刻电子显微镜观察到的小鼠骨骼肌纤维的抗体修饰的肌营养不良蛋白分子。
DOI：
10.1093/oxfordjournals.jmicro.a050883
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Shibuya
中科院分区：
文献类型：
--
作者：
Y. Wakayama;S. Shibuya
肌营养不良蛋白是Duchenne肌营养不良症基因的蛋白产物，该基因在这种遗传性疾病中是缺陷的。在这里，我们使用抗dystrophin抗体修饰的肌肉样品的快速冷冻、深度蚀刻、旋转阴影复制品，从小鼠肌纤维质膜表面的各种细胞骨架中超微结构地鉴定了dystrophin分子。该分子实际上是细胞骨架，并被结合在与质膜相关的细胞骨架的网络中。该分子似乎直接和/或通过另一个细胞骨架分子与肌动蛋白细丝相连。
Dystrophin is the protein product of Duchenne muscular dystrophy gene which is defective in this genetic disorder. Here we identified ultrastructurally the dystrophin molecule from the various cytoskeletons at the cytoplasmic surface of murine myofiber plasma membrane by using quick-freeze, deep-etch, rotary-shadow replica of anti-dystrophin antibody-decorated muscle samples. The molecule was really cytoskeleton and incorporated in the meshwork of the plasma membrane-associated cytoskeletons. The molecule appeared to connect directly and/or through another cytoskeletal molecule with actin filament.