Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas.
Transcriptomic responses to salinity stress in the Pacific oyster Crassostrea gigas.
复制标题
太平洋牡蛎对盐度胁迫的转录组反应
DOI：
10.1371/journal.pone.0046244
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Zhao X;Yu H;Kong L;Li Q
研究背景低盐度是限制海洋物种分布和生存的主要因素之一。作为广盐性物种，太平洋牡蛎巨牡蛎被认为能够耐受相对较低的盐度。利用从鳃中采集的样本对全转录组进行新一代测序，对调节巨型巨藻对渗透胁迫反应的基因进行了监测。通过 RNAseq 技术，从暴露于低盐度和最佳盐度海水的牡蛎中生成上调和下调基因的转录本目录。方法/主要发现 通过 Illumina 测序，我们报告了 1665 个上调转录本和 1815 个下调转录本。根据与已知蛋白质的序列相似性，从两组中鉴定出总共 45771 个蛋白质编码重叠群。通过GO注释和KEGG通路图谱确定，基因的功能注释恢复了不同的生物学功能和过程。根据GO注释，表达显着改变的基因在细胞过程和生物过程的调节、细胞内和细胞、结合和蛋白质结合中高度代表性。结果强调了与渗透调节、渗透应激反应的信号传导和相互作用、抗凋亡反应以及免疫反应、细胞粘附和通讯、细胞骨架和细胞周期相关的基因。结论/意义 通过超过 150 万个序列读取和两个文库的表达数据，该研究为牡蛎的信号转导途径提供了一些有用的见解，并提供了许多候选基因作为牡蛎低渗胁迫耐受性的潜在标记。此外，C. gigas转录组的表征不仅可以更好地理解牡蛎渗透压应激反应的分子机制，而且可以促进生物过程的研究，以找到海洋无脊椎动物对低渗休克的潜在生理适应。
Background Low salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the Pacific oyster Crassostrea gigas is considered to be tolerant to relative low salinity. The genes that regulate C. gigas responses to osmotic stress were monitored using the next-generation sequencing of whole transcriptome with samples taken from gills. By RNAseq technology, transcript catalogs of up- and down-regulated genes were generated from the oysters exposed to low and optimal salinity seawater. Methodology/Principal Findings Through Illumina sequencing, we reported 1665 up-regulated transcripts and 1815 down-regulated transcripts. A total of 45771 protein-coding contigs were identified from two groups based on sequence similarities with known proteins. As determined by GO annotation and KEGG pathway mapping, functional annotation of the genes recovered diverse biological functions and processes. The genes that changed expression significantly were highly represented in cellular process and regulation of biological process, intracellular and cell, binding and protein binding according to GO annotation. The results highlighted genes related to osmoregulation, signaling and interactions of osmotic stress response, anti-apoptotic reactions as well as immune response, cell adhesion and communication, cytoskeleton and cell cycle. Conclusions/Significance Through more than 1.5 million sequence reads and the expression data of the two libraries, the study provided some useful insights into signal transduction pathways in oysters and offered a number of candidate genes as potential markers of tolerance to hypoosmotic stress for oysters. In addition, the characterization of C. gigas transcriptome will not only provide a better understanding of the molecular mechanisms about the response to osmotic stress of the oysters, but also facilitate research into biological processes to find underlying physiological adaptations to hypoosmotic shock for marine invertebrates.