Assessing the reliability of fatty acid-specific stable isotope analysis for trophic studies
Assessing the reliability of fatty acid-specific stable isotope analysis for trophic studies
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DOI：
10.1111/j.2041-210x.2011.00111.x
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发表时间：
2011-12-01
影响因子：
6.6
通讯作者：
Mariotti, Andre
中科院分区：
文献类型：
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作者：
Bec, Alexandre;Perga, Marie-Elodie;Mariotti, Andre
1.脂肪酸特异性稳定同位素分析（FA-SIA）预计将涵盖使用更经典的营养标志物（如大块组织稳定同位素或脂肪酸分析）时遇到的大多数限制。然而，使用FA-SIA进行营养学研究的一个基本前提是消费者的个体FA d13 C值反映了其饮食。迄今为止，使用FA-SIA的实地研究已经做出了这一假设，这并不一定得到罕见的实验测试的支持。通过对水蚤的摄食实验，验证水蚤个体脂肪酸的d13 C值与其食物的d13 C值是否存在相关性.只有多不饱和脂肪酸（PUFA）和支链脂肪酸（BrFA）的稳定同位素组成从食物中整体传递到水蚤脂质中，然而，根据所考虑的食物来源，有显著的同位素分馏.建立了一个模型，以评估这种变化如何影响FA-SIA的可靠性，以跟踪消费者的PUFA和BrFA的饮食来源在该领域。结果表明，提供的端元是实质性的同位素不同，FA-SIA可以提供有价值的见解的途径，将这些specialFA在该领域的消费者。
1. Fatty acidspecific stable isotope analysis (FA-SIA) is expected to encompass most of the limits encountered when using more classical trophic markers such as bulk tissue stable isotope or fatty acid analyses. However, an essential premise for using FA-SIA for trophic studies is that the individual FA d13C values of the consumer reflect those of its diet. Field studies using FA-SIA have so far made this assumption, which is not necessarily supported by the rare experimental tests.2. A feeding experiment was conducted on Daphnia to test whether the d 13 C values of individual fatty acids in Daphnia were actually related to those of its food.3. Only the stable isotope composition of polyunsaturated (PUFA) and branched fatty acids (BrFA) was globally transmitted from the diet to Daphnia lipids, with however a significant isotope fractionation that varied depending on the considered diet source.4. A model was constructed to evaluate how such variability may affect the reliability of FA-SIA to track the dietary sources of consumers' PUFA and BrFA in the field. Results suggest that provided the endmembers are substantially isotopically different, FA-SIA could provide valuable insights into the pathways conveying these particularFA to consumers in the field.