Identification and Characterization of HAESA-Like Genes Involved in the Fruitlet Abscission in Litchi
Identification and Characterization of HAESA-Like Genes Involved in the Fruitlet Abscission in Litchi
复制标题
DOI：
10.3390/ijms20235945
复制
发表时间：
2019-11
影响因子：
5.6
通讯作者：
Fei Wang;Z. Zheng;Ye Yuan;Jianguo Li;Minglei Zhao
中科院分区：
文献类型：
--
作者：
Fei Wang;Z. Zheng;Ye Yuan;Jianguo Li;Minglei Zhao
由于早熟灌浆会降低作物产量，因此灌浆调节是一个重要的农业问题。在模式植物拟南芥中，开花缺乏脱落（IDA）肽及其受体HAESA（HAE）和HAESA样激酶2（HSL 2）是控制花器官脱落的核心组分。然而，在非模式植物中IDA-HAE/HSL 2的同源物是否与凋亡相关仍不清楚。在此之前，我们发现LcIDL 1，一个来自荔枝的IDA的同源物，在拟南芥花器官发育的控制中具有与AtIDA相似的作用。在此，我们进一步分离了一个类似HAESA的同源物LcHSL 2，它可能与荔枝的幼果脱落有关。LcHSL 2在野生型拟南芥中的异位表达对花器官的发育没有影响。然而，在hae hsl 2突变体背景下，它的存在完全挽救了花器官脱落缺陷。LcHSL 2定位于细胞膜上，并且LcHSL 2基因在荔枝的花梗分裂区（AZ）和拟南芥的花AZ中表达。实时荧光定量PCR分析表明，在乙烯利诱导的荔枝幼果脱落过程中，LcHSL 2的表达量增加。综上所述，我们的研究结果表明，HSL 2同源物在拟南芥和荔枝中具有功能保守性，LcHSL 2可能在荔枝幼果脱落的调控中起关键作用。
Regulation of abscission is an important agricultural concern since precocious abscission can reduce crop yield. INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) peptide and its receptors the HAESA (HAE) and HAESA-like2 (HSL2) kinases have been revealed to be core components controlling floral organ abscission in the model plant Arabidopsis. However, it is still unclear whether the homologs of IDA-HAE/HSL2 in non-model plants are correlated to abscission. Previously, we found LcIDL1, a homolog of IDA from litchi, has a similar role to AtIDA in control of floral organ abscission in Arabidopsis. Here, we further isolated an HAESA-like homolog, LcHSL2, which is likely involved in the fruitlet abscission in litchi. Ectopic expression of LcHSL2 in wild type Arabidopsis has no effect on the floral organ abscission. However, its presence in the hae hsl2 mutant background completely rescued the floral organ abscission deficiency. LcHSL2 is localized in the cell membrane and the LcHSL2 gene is expressed at the pedicel abscission zone (AZ) of litchi and floral AZ of Arabidopsis. Real-time PCR analysis showed that the expression level of LcHSL2 was increased during ethephon-induced fruitlet abscission in litchi. Taken together, our findings suggest that HSL2 homologs have functional conservation in Arabidopsis and litchi, and LcHSL2 might play a critical role in regulation of fruitlet abscission in litchi.