Molecular cloning and expression analysis of PDR1-like gene in ginseng subjected to salt and cold stresses or hormonal treatment.
Molecular cloning and expression analysis of PDR1-like gene in ginseng subjected to salt and cold stresses or hormonal treatment.
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DOI：
10.1016/j.plaphy.2013.07.011
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发表时间：
2013-10
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通讯作者：
Ru Zhang;Jie Zhu;Hongzhe Cao;Y. An;Jingjia Huang;Xianghui Chen;Nuruzzaman Mohammed;S. Afrin;Zhi-Yong Luo
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文献类型：
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作者：
Ru Zhang;Jie Zhu;Hongzhe Cao;Y. An;Jingjia Huang;Xianghui Chen;Nuruzzaman Mohammed;S. Afrin;Zhi-Yong Luo
植物多效抗药性（PDR）家族的ATP结合盒（ABC）转运蛋白可能参与多种生物学过程。目前，人们对它们的实际生理功能知之甚少。克隆了人参PDR转运蛋白基因（PgPDR 1），其cDNA开放阅读框为4344 bp。推导的氨基酸序列包含PDR转运蛋白的特征性结构域：步行者A、步行者B和ABC签名。基因组DNA杂交分析表明，人参中存在一个拷贝的PgPDR 1基因。亚细胞定位结果表明，PgPDR 1-GFP融合蛋白特异性定位于细胞膜。启动子区分析表明，顺式作用元件的存在，其中一些是puronectin参与响应激素，光和压力。为了了解PgPDR 1的功能作用，我们研究了PgPDR 1在不同组织和不同条件下的表达模式。实时荧光定量PCR（qRT-PCR）和Western blotting分析表明，PgPDR 1在根和叶中的表达量高于种子和茎。水杨酸（SA）和低温处理可上调PgPDR 1的表达，阿坝可下调PgPDR 1的表达，MeJA在转录水平和蛋白水平对PgPDR 1的表达有不同程度的调控。这些结果表明PgPDR 1可能参与了对环境应激和激素的应答。
The plant pleiotropic drug resistance (PDR) family of ATP-binding cassette (ABC) transporters is potentially involved in diverse biological processes. Currently, little is known about their actual physiological functions. A Panax ginseng PDR transporter gene (PgPDR1) was cloned and the cDNA has an open reading frame of 4344 bp. The deduced amino acid sequence contained the characteristic domains of PDR transporters: Walker A, Walker B, and ABC signature. Genomic DNA hybridization analysis indicated that one copy ofPgPDR1gene was present inP. ginseng. Subcellular localization showed that PgPDR1-GFP fusion protein was specifically localized in the cell membrane. Promoter region analysis revealed the presence of cis-acting elements, some of which are putatively involved in response to hormone, light and stress. To understand the functional roles ofPgPDR1, we investigated the expression patterns ofPgPDR1in different tissues and under various conditions. Quantitative real-time PCR (qRT-PCR) and Western blotting analysis showed thatPgPDR1was expressed at a high level in the roots and leaves compared to seeds and stems. The expression ofPgPDR1was up-regulated by salicylic acid (SA) or chilling, down-regulated by ABA, and regulated differently at transcript and protein levels by MeJA. These results suggest thatPgPDR1might be involved in responding to environmental stresses and hormones.