Highly sensitive non-enzymatic glucose sensor based on carbon nanotube microelectrode set
Highly sensitive non-enzymatic glucose sensor based on carbon nanotube microelectrode set
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DOI：
10.1016/j.snb.2021.130688
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发表时间：
2021-09-17
影响因子：
8.4
通讯作者：
Alvarez, Noe T.
中科院分区：
文献类型：
--
作者：
Gupta, Pankaj;Gupta, Vandna K.;Alvarez, Noe T.
在这项工作中，碳纳米管微电极设置（CNT μ ES）进行了修改，其中三个电极的基础上高度致密的碳纳米管纤维（HD-CNTf）的横截面（长度~ 40 μ m）嵌入在一个惰性的聚合物基质与暴露的开放式CNT在界面处。用铜纳米颗粒（CuNPs）电化学修饰的HD-CNTf横截面（~ 40 μ m直径）用作工作电极;裸HDCNTf横截面（~ 94 μ m直径）用作对电极;用Ag/AgCl电镀然后用NafionTM涂覆的HD-CNTf横截面（~ 94 μ m直径）用作准参比电极。在0.1 M NaOH溶液中，用循环伏安法和安培法研究了CuNPs/HD-CNTf微传感器对葡萄糖电氧化的电化学活性。如电流分析法研究所示，所提出的非酶CuNPs/HD-CNTf微传感器具有显著低的检测限（28 nM）和宽的线性定量范围，具有优异的灵敏度（1942 nA.mu M- 1.cm- 2）。这种敏感性可以归因于电催化CuNP和对准的HD-CNTf的协同效应，其提供优异的导电性。葡萄糖在该微传感器上的电氧化不受氯离子的毒害，且与氧浓度无关。开发的微传感器显示，常见干扰物质和碳水化合物在其生理浓度下的氧化对干扰微不足道。最后，CuNPs/ HD-CNTf微传感器成功地应用于正常人血清和糖尿病患者尿样中葡萄糖的定量，证明了这种策略对商业非酶葡萄糖传感器的适用性。
In this work, a carbon nanotube microelectrode set (CNT mu-ES) was modified, in which three electrodes based on highly densified carbon nanotube fiber (HD-CNTf) cross-sections (length -40 mu m) were embedded in an inert polymer matrix with exposed open-ended CNTs at the interface. An HD-CNTf cross-section (-40 mu m diameter) electrochemically modified with copper nanoparticles (CuNPs) was used as the working electrode; a bare HDCNTf cross-section (-94 mu m diameter) was used as the counter electrode; and an HD-CNTf cross-section (-94 mu m diameter) electroplated with Ag/AgCl and then coated with NafionTM was used as a quasi-reference electrode. The electrochemical activity of the CuNPs/HD-CNTf microsensor for glucose electrooxidation was examined by cyclic voltammetry and amperometry in 0.1 M NaOH solution. As shown by amperometry studies, the proposed nonenzymatic CuNPs/HD-CNTf microsensor had a remarkably low limit of detection (28 nM) and a wide linear quantification range with an excellent sensitivity (1942 nA.mu M- 1.cm- 2). This sensitivity can be attributed to the synergetic effect of electrocatalytic CuNPs and aligned HD-CNTf, which provide excellent conductivity. The electrooxidation of glucose on the developed microsensor was free from chloride poisoning and independent of the oxygen concentration. The developed microsensor demonstrated insignificant interference from the oxidation of common interfering species and carbohydrate compounds at their physiological concentrations. Finally, the CuNPs/ HD-CNTf microsensor was successfully applied for the quantification of glucose in normal human serum and diabetic patient urine samples, demonstrating the applicability of this strategy for commercial nonenzymatic glucose sensors.