MADS-box gene evolution beyond flowers: expression in pollen, endosperm, guard cells, roots and trichomes
MADS-box gene evolution beyond flowers: expression in pollen, endosperm, guard cells, roots and trichomes
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DOI：
10.1046/j.1365-313x.2000.00891.x
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发表时间：
2000-11-01
期刊：
影响因子：
7.2
通讯作者：
Yanofsky, MF
中科院分区：
文献类型：
--
作者：
Alvarez-Buylla, ER;Liljegren, SJ;Yanofsky, MF
MADS盒基因编码参与植物发育的各个方面的转录调节因子。本文描述了拟南芥中5个新的MADS盒基因AGL16、AGL18、AGL19、AGL27和AGL31的克隆及其mRNA时空表达模式。这些基因可能成为植物结构进化和功能分析的重要分子工具。我们将我们的数据和以前的表达模式映射到一个新的MADS盒基因组上。这些分析表明，MADS盒家族的进化涉及快速和同时的功能多样化的营养以及生殖结构。假设的祖先基因有更广泛的表达模式比更多的衍生，已增选为假定的专门功能，建议他们的表达模式。AGL27和AGL31与最近描述的开花时间基因FLC（以前称为AGL25）密切相关，在大多数植物组织中表达。AGL19特异性表达于根分生组织的外层（侧根帽和表皮）和成熟根的中央圆柱细胞中。AGL18，这是最相似的胚胎表达的AGL15基因的序列，在胚乳和发育中的雄性和雌性配子体中表达，这表明AGL18的作用是不同于以前的MADS盒基因。最后，AGL16 RNA积累在叶片保卫细胞和毛状体中。我们的新的单系分化揭示了7个新的单系分支的MADS盒序列不特定的花，这表明复杂的调控网络，涉及几个MADS盒基因，类似于那些控制花的发育，营养结构的发展。
MADS-box genes encode transcriptional regulators involved in diverse aspects of plant development. Here we describe the cloning and mRNA spatio-temporal expression patterns of five new MADS-box genes from Arabidopsis: AGL16, AGL18, AGL19, AGL27 and AGL31. These genes will probably become important molecular tools for both evolutionary and functional analyses of vegetative structures. We mapped our data and previous expression patterns onto a new MADS-box phylogeny. These analyses suggest that the evolution of the MADS-box family has involved a rapid and simultaneous functional diversification in vegetative as well as reproductive structures. The hypothetical ancestral genes had broader expression patterns than more derived ones, which have been co-opted for putative specialized functions as suggested by their expression patterns. AGL27 and AGL31, which are closely related to the recently described flowering-time gene FLC (previously AGL25), are expressed in most plant tissues. AGL19 is specifically expressed in the outer layers of the root meristem (lateral root cap and epidermis) and in the central cylinder cells of mature roots. AGL18, which is most similar in sequence to the embryo-expressed AGL15 gene, is expressed in the endosperm and in developing male and female gametophytes, suggesting a role for AGL18 that is distinct from previously characterized MADS-box genes. Finally, AGL16 RNA accumulates in leaf guard cells and trichomes. Our new phylogeny reveals seven new monophyletic clades of MADS-box sequences not specific to flowers, suggesting that complex regulatory networks involving several MADS-box genes, similar to those that control flower development, underlie development of vegetative structures.