Bone Imaging: Osteoclast and Osteoblast Dynamics
Bone Imaging: Osteoclast and Osteoblast Dynamics
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DOI：
10.1007/978-1-4939-7762-8_1
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Ishii, Masaru
中科院分区：
文献类型：
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作者：
Kikuta, Junichi;Ishii, Masaru
骨由骨吸收破骨细胞和成骨细胞持续重塑。虽然长期以来人们一直认为破骨细胞和成骨细胞之间的通讯严格调控着骨骼的动态平衡，但基本的过程和动力学仍然难以捉摸。为了解决这一问题，我们建立了活体骨成像系统，使用多光子显微镜来显示活骨中成熟的破骨细胞和成骨细胞。本文描述了利用活体多光子显微镜可视化活骨组织中骨吸收破骨细胞和成骨细胞的体内行为的方法。这种方法有助于在体内研究骨质疏松和类风湿性关节炎等骨质破坏疾病的发病机制中的细胞动力学，因此将有助于评估新型抗骨吸收药物的疗效。
Bone is continually remodeled by bone-resorbing osteoclasts and bone-forming osteoblasts. Although it has long been believed that bone homeostasis is tightly regulated by communication between osteoclasts and osteoblasts, the fundamental process and dynamics have remained elusive. To resolve this, we established an intravital bone imaging system using multiphoton microscopy to visualize mature osteoclasts and osteoblasts in living bone.We herein describe the methodology for visualizing the in vivo behavior of bone-resorbing osteoclasts and bone-forming osteoblasts in living bone tissues using intravital multiphoton microscopy. This approach facilitates investigation of cellular dynamics in the pathogenesis of bone-destructive disorders, such as osteoporosis and rheumatoid arthritis in vivo, and would thus be useful for evaluating the efficacy of novel anti-bone-resorptive drugs.