DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India.
DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India.
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DOI：
10.1186/s13071-016-1722-z
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发表时间：
2016-08-22
影响因子：
3.2
通讯作者：
Carpenter S
中科院分区：
文献类型：
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作者：
Harrup LE;Laban S;Purse BV;Reddy YK;Reddy YN;Byregowda SM;Kumar N;Purushotham KM;Kowalli S;Prasad M;Prasad G;Bettis AA;De Keyser R;Logan J;Garros C;Gopurenko D;Bellis G;Labuschagne K;Mathieu B;Carpenter S
库蠓传播蓝舌病毒（BTV），蓝舌病是反刍动物一种重要的经济疾病。在印度南部，BT的高地方性暴发使该地区自给自足的农民付出了高昂的代价，影响了绵羊生产。有效的库蠓监测方法与准确的物种鉴定相结合，可以加快反应速度，最大限度地减少BT暴发。在此，我们评估了采样方法和DNA条形码在印度南部库蠓检测和鉴定中的效用，以便为该地区未来的种群监测提供信息基础。基于线粒体细胞色素c氧化酶I （COI）基因DNA条形码区域的序列比较，并达到生命条形码计划定义的质量标准，利用采集自泰米尔纳德邦和卡纳塔克邦的库蠓构建了未来监测形态鉴定的框架。2013年11月，在泰米尔纳德邦金奈（Chennai）的一个地点，对绿色（570 nm）和紫外线（UV） （390 nm）发光二极管（LED）吸盘捕集器的成对捕获量进行了20晚采样，比较了其多样性和丰度。库蠓的DNA条形码序列与国外已有的DNA条形码数据和主要媒介种的形态鉴定基本一致。然而，在某些类群中存在着隐种多样性的序列差异，有待进一步研究。虽然紫外线LED疾病控制中心（CDC）诱捕器收集的物种多样性与绿色LED CDC诱捕器收集的物种多样性没有显著差异，但紫外线CDC诱捕器收集的库蠓个体数量明显优于绿色LED CDC诱捕器。在印度南部，库蠓样本的大多数潜在病媒物种的形态学鉴定似乎相对稳健；然而，在一些类群中存在潜在的隐种多样性，需要进一步调查。建议在印度南部使用UV LED CDC诱捕器监测库蠓。本文的在线版本（doi:10.1186/s13071-016-1722-z）包含补充材料，可供授权用户使用。
Culicoides spp. biting midges transmit bluetongue virus (BTV), the aetiological agent of bluetongue (BT), an economically important disease of ruminants. In southern India, hyperendemic outbreaks of BT exert high cost to subsistence farmers in the region, impacting on sheep production. Effective Culicoides spp. monitoring methods coupled with accurate species identification can accelerate responses for minimising BT outbreaks. Here, we assessed the utility of sampling methods and DNA barcoding for detection and identification of Culicoides spp. in southern India, in order to provide an informed basis for future monitoring of their populations in the region. Culicoides spp. collected from Tamil Nadu and Karnataka were used to construct a framework for future morphological identification in surveillance, based on sequence comparison of the DNA barcode region of the mitochondrial cytochrome c oxidase I (COI) gene and achieving quality standards defined by the Barcode of Life initiative. Pairwise catches of Culicoides spp. were compared in diversity and abundance between green (570 nm) and ultraviolet (UV) (390 nm) light emitting diode (LED) suction traps at a single site in Chennai, Tamil Nadu over 20 nights of sampling in November 2013. DNA barcode sequences of Culicoides spp. were mostly congruent both with existing DNA barcode data from other countries and with morphological identification of major vector species. However, sequence differences symptomatic of cryptic species diversity were present in some groups which require further investigation. While the diversity of species collected by the UV LED Center for Disease Control (CDC) trap did not significantly vary from that collected by the green LED CDC trap, the UV CDC significantly outperformed the green LED CDC trap with regard to the number of Culicoides individuals collected. Morphological identification of the majority of potential vector species of Culicoides spp. samples within southern India appears relatively robust; however, potential cryptic species diversity was present in some groups requiring further investigation. The UV LED CDC trap is recommended for surveillance of Culicoides in southern India. The online version of this article (doi:10.1186/s13071-016-1722-z) contains supplementary material, which is available to authorized users.