DEVELOPMENTAL-CHANGES IN THE LARVAL FAT-BODY DURING METAMORPHOSIS IN GALLERIA-MELLONELLA
DEVELOPMENTAL-CHANGES IN THE LARVAL FAT-BODY DURING METAMORPHOSIS IN GALLERIA-MELLONELLA
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DOI：
10.1016/0022-1910(86)90005-3
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发表时间：
1986-01-01
影响因子：
2.2
通讯作者：
KUMARAN, AK
中科院分区：
文献类型：
--
作者：
GRZELAK, K;KUMARAN, AK
研究了正常、激素和/或20-羟基蜕皮激素处理的mellonella末龄幼虫脂肪体中丰富类mRNA群体的表观RNA合成率和发育变化。在这一快速生长期，摄食幼虫总RNA和聚(A) RNA的表观合成率基本保持不变。从不同发育阶段mrna的体外翻译产物的电泳分析来看，mrna可分为三大类。大多数丰富的mrna所属的第一类在整个体育场中几乎保持相同的相对丰度。第二类mrna在晚期幼虫中比在老年幼虫中更丰富，并且在变质蜕皮之前无法检测到，例如编码47.5,40和21千道尔顿多肽的mrna。第三类mrna在1 d末龄幼虫脂肪体中未检测到，但在体育场中较晚出现，如编码80和23千道尔顿多肽的mrna。在幼体激素诱导多余的幼虫蜕皮时，在蜕皮前通常会消失的第二类mrna持续存在。20-羟基蜕皮激素的使用导致了过早蜕皮，诱导了编码50.5千道尔顿多肽的聚(A) RNA的产生，也导致了一些mrna的过早消失，例如编码47.5和40千道尔顿多肽的mrna。然而，在应用表皮甾体激素时，幼代激素的存在仅抑制编码50.5千道尔顿多肽的mRNA的产生。
The apparent rates of RNA synthesis and developmental changes in the abundant class of mRNA populations were studied in the fat body of normal, juvenile hormone- and/or 20-hydroxyecdysone-treated last-instar larvae of Galleria mellonella. The apparent rates of total and poly (A) RNA syntheses in the feeding larvae remained more or less unchanged during this period of rapid growth. From an electrophoretic analysis of the in vitro translation products of the mRNAs from diverse developmental stages, the mRNAs could be divided into three major categories. The first category to which a majority of the abundant class of mRNAs belong remained at nearly the same relative abundance all through the stadium. The second category of mRNAs was more abundant in early last-instar larvae than in older larvae and was undetectable just before the metamorphic moult, e.g. those coding for 47.5, 40 and 21 kdalton polypeptides. The third category of mRNAs was not detectable in day-1 last-instar larval fat body but appeared later in the stadium, e.g. mRNAs coding for 80 and 23 kdalton polypeptides. On induction of a supernumerary larval moult by juvenile hormone the second category of mRNAs, which would normally disappear prior to a metamorphic moult, persisted. Application of 20-hydroxyecdysone, which initiates precocious moult, induced production of poly (A) RNA that codes for a 50.5 kdalton polypeptide, and also caused precocious disappearance of some mRNAs, e.g. those coding for 47.5 and 40 kdalton polypeptides. However, the presence of juvenile hormone at the time of application of the ecdysteroid inhibited only production of the mRNA coding for the 50.5 kdalton polypeptide.