Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control
Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control
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DOI：
10.1016/j.jpor.2014.06.003
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发表时间：
2014-10-01
影响因子：
3.6
通讯作者：
Kuboki, Takuo
中科院分区：
文献类型：
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作者：
Minakuchi, Hajime;Sogawa, Chiharu;Kuboki, Takuo
目的：本研究的目的是评估睡眠磨牙症（SB）频率与血小板5-羟色胺转运蛋白（SERT）驱动的5-羟色胺（5-HT）摄取之间的相关性。受试者是牙科实习住院医师和冈山大学医院的教职员工，他们知道自己患有严重的SB或没有SB。SB频率通过独立的肌电图检测器/分析器连续3个晚上进行评估，其将个体SB水平指示为四个等级（评分0、1、2和3）之一。当SB评分在3个晚上仅为0或1时，受试者被归类为正常对照（NC），或当评分为2或3时被归类为重度SB。在3个晚上期间分数在0到3之间波动的那些受试者从进一步分析中省略。在最终SB评估后的早晨采集空腹外周静脉血样。从外周血小板收集的SERTs蛋白的量进行定量，并使用[H-3]-5-HT.Results的摄取测定SERTs转运活性进行了评估：13个严重SB受试者和7个NC受试者是合格的。性别分布、平均年龄、5-HT浓度和血小板中SERT蛋白总量在NC和重度SB之间无显著差异（p = 0.85：卡方检验; p = 0.64，0.26，0.46：t检验）。然而，与严重SB受试者相比，NC中血小板对[H-3]-5-HT的摄取显著更高（12.79 +/- 1.97，8.27 +/- 1.91 fmol/10（5）血小板/min，p < 0.001，t检验）。(C)2014年日本口腔修复学会。由Elsevier爱尔兰出版。All rights reserved.
Purpose: The aim of this study was to evaluate the correlation between sleep bruxism (SB) frequency and serotonin transporter (SERT)-driven serotonin (5-HT)-uptake in platelets.Methods: Subjects were dental trainee residents and faculty members of Okayama University Hospital who were aware of having severe or no SB. SB frequency was assessed for 3-consecutive nights by a self-contained electromyographic detector/analyzer, which indicated individual SB levels as one of four grades (score 0, 1, 2 and 3). Subjects were classified as normal control (NC) when SB scores indicated only 0 or 1 during the 3 nights, or as severe SB for scores 2 or 3. Those subjects whose scores fluctuated from 0 to 3 during the 3 nights were omitted from further analysis. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. Amounts of SERTs proteins collected from peripheral platelets were quantified using ELISA, and SERTs transport activity was assessed by uptake assay using [H-3]-5-HT.Results: Thirteen severe SB subjects and 7 NC subjects were eligible. Gender distribution, mean age, 5-HT concentration and total amounts of SERT protein in platelets showed no significant differences between NC and severe SB (p = 0.85: Chi-squared test; p = 0.64, 0.26, 0.46: t-test). However, [H-3]-5-HT uptake by platelets was significantly greater in NC compared to severe SB subjects (12.79 +/- 1.97, 8.27 +/- 1.91 fmol/10(5) platelets/min, p < 0.001, t-test).Conclusion: The results of this pilot study suggest a possible correlation between peripheral platelet serotonin transporter uptake ability and SB severity. (C) 2014 japan Prosthodontic Society. Published by Elsevier Ireland. All rights reserved.