Effects of inhibitors of glycoprotein processing on oligodendroglial differentiation in primary cultures of embryonic rat brain cells.
Effects of inhibitors of glycoprotein processing on oligodendroglial differentiation in primary cultures of embryonic rat brain cells.
复制标题
糖蛋白加工抑制剂对胚胎大鼠脑细胞原代培养物中少突胶质细胞分化的影响。
DOI：
10.1002/jnr.490200203
复制
发表时间：
1988
影响因子：
4.2
通讯作者：
Bhat,NR
中科院分区：
文献类型：
--
作者：
Bhat,NR
在胚胎大鼠脑细胞培养物中检查了N-连接寡糖加工抑制剂对少突胶质细胞分化的影响。发现葡萄糖苷酶抑制剂1-脱氧野尻霉素（dNM）和栗精胺对少突胶质细胞特性的发育表达具有显着的抑制作用，即，磺基半乳糖脂合成和2′3′-环核苷酸3′-磷酸水解酶（CNP）。另一方面，甘露糖苷酶抑制剂1-脱氧甘露尻霉素（dMM）和苦马豆素的作用相对较小。由于这两类抑制剂都能阻断糖肽的伴刀豆球蛋白A-琼脂糖亲和色谱显示的复合型寡糖链的形成，因此复合寡糖似乎在少突胶质细胞分化中不起作用。相反，结果表明，涉及通过加工葡糖苷酶去除葡萄糖残基的早期修剪反应可能对少突胶质细胞分化所必需的特定糖蛋白的功能至关重要。
The effects of N‐linked oligosaccharide processing inhibitors on oligodendroglial differentiation were examined in cultures of embryonic rat brain cells. The glucosidase inhibitors, 1‐deoxynojirimycin (dNM) and castanospermine, were found to have marked inhibitory effects on the developmental expression of oligodendroglial properties, i.e., sulfogalactolipid synthesis and 2′3′‐cyclic nucleotide 3′‐phosphohydrolase (CNP). On the other hand, the mannosidase inhibitors, 1‐deoxymannojirimycin (dMM) and swainsonine, had relatively little effects. Since both classes of inhibitors block the formation of complex‐type oligosaccharide chains as revealed by concanavalin A‐Sepharose affinity chromatography of the glycopeptides, complex oligosaccharides oligosaccharides do not seem to play a role in oligodendroglial differentiation. The results indicate instead that the early trimming reactions involving the removal of glucose residues by processing glucosidases may be critical for the functioning of specific glycoprotein(s) essential to oligodendroglial differentiation.