Cyto- and Chemoarchitecture of the Cerebellum of the Short-Beaked Echidna (Tachyglossus aculeatus)
Cyto- and Chemoarchitecture of the Cerebellum of the Short-Beaked Echidna (Tachyglossus aculeatus)
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短喙针鼹（Tachyglossus aculeatus）小脑的细胞和化学结构
DOI：
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
C. Watson
中科院分区：
文献类型：
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作者：
K. Ashwell;G. Paxinos;C. Watson
单孔目动物（针鼹和鸭嘴兽）的小脑在大体形态学和显微解剖学上被一些作者认为具有“鸟类”或“爬行动物”的特征。我们已经使用尼氏染色结合酶组织化学乙酰胆碱酯酶和细胞色素氧化酶和免疫组织化学非磷酸化神经丝蛋白（SMI-32抗体），钙结合蛋白（小清蛋白，钙结合蛋白和钙视网膜蛋白）和酪氨酸羟化酶检查的细胞和化学结构的小脑皮质和小脑深部核的短喙针鼹。非磷酸化神经丝（SMI-32抗体）的免疫反应性被发现在小脑深核和浦肯野细胞的大部分地区，除了结节。用SMI-32免疫反应性鉴定的浦肯野细胞在形态上明显是哺乳动物的。小清蛋白和钙结合蛋白免疫反应性被发现在浦肯野细胞的染色强度和浦肯野细胞轴突穿过小脑白色物质或终止于Lugaro细胞的一些区域变化。钙结合蛋白免疫反应也存在于下橄榄复合体神经元。桥小脑纤维和袢状小叶深颗粒细胞层的小细胞内均可见钙视黄蛋白免疫反应阳性。我们发现，虽然小脑深核的划分比啮齿动物小脑清楚得多，但在针鼹中可以区分内侧、间置和外侧核成分。根据我们的技术所能确定的，针鼹鼠的小脑显示出所有与兽目哺乳动物小脑相似的大体和细胞学特征。
The monotremes (echidnas and platypus) have been claimed by some authors to show ‘avian’ or ‘reptilian’ features in the gross morphology and microscopic anatomy of the cerebellum. We have used Nissl staining in conjunction with enzyme histochemistry to acetylcholinesterase and cytochrome oxidase and immunohistochemistry to non-phosphorylated neurofilament protein (SMI-32 antibody), calcium binding proteins (parvalbumin, calbindin and calretinin) and tyrosine hydroxylase to examine the cyto- and chemoarchitecture of the cerebellar cortex and deep cerebellar nuclei in the short-beaked echidna. Immunoreactivity for non-phosphorylated neurofilament (SMI-32 antibody) was found in the deep cerebellar nuclei and in Purkinje cells of most regions except the nodule. Purkinje cells identified with SMI-32 immunoreactivity were clearly mammalian in morphology. Parvalbumin and calbindin immunoreactivity was found in Purkinje cells with some regional variation in staining intensity and in Purkinje cell axons traversing cerebellar white matter or terminating on Lugaro cells. Calbindin immunoreactivity was also present in inferior olivary complex neurons. Calretinin immunoreactivity was found in pontocerebellar fibers and small cells in the deep granule cell layer of the ansiform lobule. We found that, although the deep cerebellar nuclei were much less clearly demarcated than in the rodent cerebellum, it was possible to distinguish medial, interposed and lateral nuclear components in the echidna. As far as we can determine from our techniques, the cerebellum of the echidna shows all the gross and cytological features familiar from the cerebellum of therian mammals.
DOI：
10.1073/pnas.84.10.3472
发表时间：
1987-05-01
影响因子：
11.1
作者：
HOFFMAN, PN;CLEVELAND, DW;PRICE, DL
通讯作者：
PRICE, DL