DNA OF LYMPHOMA-ASSOCIATED HERPESVIRUS (HVMF1) IN SIV-INFECTED MONKEYS (MACACA-FASCICULARIS) SHOWS HOMOLOGIES TO EBNA-1, EBNA-2 AND EBNA-5 GENES
DNA OF LYMPHOMA-ASSOCIATED HERPESVIRUS (HVMF1) IN SIV-INFECTED MONKEYS (MACACA-FASCICULARIS) SHOWS HOMOLOGIES TO EBNA-1, EBNA-2 AND EBNA-5 GENES
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DOI：
10.1002/ijc.2910590223
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发表时间：
1994-10-15
影响因子：
6.4
通讯作者：
ERNBERG, I
中科院分区：
文献类型：
--
作者：
LI, SL;BIBERFELD, P;ERNBERG, I
我们鉴定了一种与SIV感染的食蟹猴淋巴瘤相关的新的EB病毒样疱疹病毒，并建议将这种病毒命名为恒河猴疱疹病毒1(HVMF1)。采用Southern杂交、聚合酶链式反应和测序等方法对HVMF1中具有潜在致癌意义的基因组区域进行分析，并与人EBV DNA进行比较。来自7个SIV相关淋巴瘤和1个淋巴瘤来源的细胞株的病毒与EBV的EBNA1和EBNA2编码区有同源性，而与EBV-LMP1的一些同源性仅在低严格杂交中检测到。在HVMF1中还可以发现与长内部重复序列(IR1；BamHI W)、EBER1和2以及DNA复制的潜在起点(ORIP)的同源区。除IR1外，其余编码区的限制性内切酶图谱与EBV不同。HVMF1 DNA外显子W1和W2的EBNA5同源区与EBV外显子W1和W2的同源性为65%，与包括内含子在内的整个区域的同源性为80%。由于EBNA5已被报道在体外结合肿瘤抑制蛋白P53和Rb，HVMF1的同源性可能对这种猴子疱疹病毒的淋巴生成能力起重要作用。(C)1994年Wiley-Liss，Inc.
We have characterized a new Epstein-Barr-virus(EBV)-like herpesvirus associated with lymphomas of SIV-infected cynomolgus (Macaca fascicularis) monkeys and propose that this virus is designated herpesvirus macaca fascicularis 1 (HVMF1). genomic regions in HVMF1 of potential significance for tumor pathogenesis were analyzed by Southern blotting, PCR and sequencing, and compared with human EBV DNA. Virus from 7 SIV-associated lymphomas and one lymphoma-derived cell line were shown to share homology with the EBNA1- and EBNA2- coding regions of EBV, while some homology to EBV-LMP1 was detectable only at low-stringency hybridization. Homologous regions to the long internal repeat (IR1;BamHI W), the EBER1 and 2 and the latent origin of DNA replication (oriP) could also be demonstrated in HVMF1. These coding regions, except IR1, showed restriction-enzyme maps different from those of EBV. Sequencing of the EBNA5 homologous region of HVMF1 DNA, corresponding to exons W1 and W2, showed 65% homology to the EBV exons W1 and W2, and 80% to the whole region including the intron. Since EBNA5 has been reported to bind tumor-suppressor proteins p53 and Rb in vitro, the HVMF1 homology could be important for the lymphomagenic capacity of this monkey herpesvirus. (C) 1994 Wiley-Liss, Inc.