THE SSS GENE-PRODUCT, WHICH AFFECTS PYOVERDIN PRODUCTION IN PSEUDOMONAS-AERUGINOSA 7NSK2, IS A SITE-SPECIFIC RECOMBINASE
THE SSS GENE-PRODUCT, WHICH AFFECTS PYOVERDIN PRODUCTION IN PSEUDOMONAS-AERUGINOSA 7NSK2, IS A SITE-SPECIFIC RECOMBINASE
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DOI：
10.1111/j.1365-2958.1994.tb01335.x
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发表时间：
1994-12-01
影响因子：
3.6
通讯作者：
MERGEAY, M
中科院分区：
文献类型：
--
作者：
HOFTE, M;DONG, QG;MERGEAY, M
Pyoverdin production by Pseodomonas aeruginosa strain 7NSK2 was induced by Zn(ll) in the presence of iron. A mutant was isolated in which Zn(ll) no longer induced pyoverdin production. The sss gene which was inactivated in this mutant was cloned and sequenced. Its protein sequence showed 50% identity to the XerC protein of Escherichia coli, which is a member of the lambda integrase family of site-specific recombinases, An open reading frame was found upstream of sss whose protein sequence showed strong identity to DapF, the diaminopimelate epimerase. In E. coli, xerC is part of a multicistronic unit that also contains dapF, The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coli xerC mutant and the E. coli xerC gene could complement a genomic sss mutation in P. aeruginosa.
