Metabolism and renal handling of sodium arsenate in dogs.
Metabolism and renal handling of sodium arsenate in dogs.
复制标题
犬砷酸钠的代谢和肾脏处理。
DOI：
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发表时间：
1983
影响因子：
1
通讯作者：
S. Peoples
中科院分区：
文献类型：
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作者：
H. Tsukamoto;H. Parker;S. Peoples
在5只狗身上研究了砷酸钠的肾脏处理。静脉注射小剂量(0.73 mg/kg体重)的亚砷酸钠，可在血浆和尿液中检测到不同浓度的亚砷酸盐。在中等剂量(7.33 mg/kg)下，肾小管上皮细胞可能是砷的重吸收、亚砷还原为亚砷酸盐、以及后者分泌或扩散到尿中的场所。然而，使用高剂量(14.66 mg/kg)，尽管亚砷酸盐还原为亚砷酸盐的模式类似，但亚砷酸盐在血浆中发生了显著的再吸收，而不是分泌或扩散到尿液中。由于重吸收，血浆中亚砷酸盐的含量(占总砷的18.4+/-3.5%)比中剂量实验中测得的(6.0+/-1.0%)高约3倍。在持续110分钟的清除实验中，只有40%~45%的砷从尿液中排出，并检测到微量的二甲基砷酸(DMAA)。相比之下，到了第二天，DMAA是尿液中排出的主要代谢物。AS和DMAA的这种排泄部分是由于55%到60%的延迟排泄，因为它储存在体内并随后代谢。
Renal handling of sodium arsenate was studied in 5 dogs. Using a low dose (0.73 mg/kg of body weight) of sodium arsenate given IV, variable arsenite concentrations were detected in plasma and urine. Using a medium dose (7.33 mg/kg), the renal tubule cells were determined to be the probable sites of reabsorption of arsenate, reduction of arsenate to arsenite, and secretion or diffusion of the latter into urine. However, using a high dose (14.66 mg/kg), despite a similar pattern of reduction of arsenate to arsenite, marked reabsorption of arsenite into plasma took place instead of secretion or diffusion into urine. Because of reabsorption, the amount of arsenite in plasma (18.4 +/- 3.5% of the total As) was about 3 times higher than that measured during the medium dose experiment (6.0 +/- 1.0%). During the clearance experiments which lasted 110 minutes, only 40% to 45% of the arsenate infused was excreted in urine, and a minimal amount of dimethylarsinic acid (DMAA) was detected. In contrast, by the next day, DMAA was the major metabolite excreted in urine. This excretion of As as DMAA was partly due to delayed excretion of 55% to 60% As that was stored in the body and was subsequently metabolized.