Enzymatic repair of an expanded genetic information system
Enzymatic repair of an expanded genetic information system
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DOI：
10.1093/nar/gkg709
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发表时间：
2003-09-01
影响因子：
14.9
通讯作者：
Prudent, JR
中科院分区：
文献类型：
--
作者：
Moser, MJ;Prudent, JR
嗜热细菌的切除修复机制已被证明可以识别和修复扩展的遗传碱基对。天然水生栖热菌DNA聚合酶将去除错配的天然碱基，并用非天然碱基替换它以形成扩展的碱基对。此外，DNA连接酶将识别由聚合酶在两个非天然碱基对之间形成的切口，并共价连接两条链，从而证明分叉的碱基配对模型双链体的完全修复。这些结果进一步证明了这样一种观点，即含有扩展遗传字母表的生物体的细胞复制和修复机制可以识别并正确修复含有连续非自然碱基的位点。
The excision repair machinery of a thermophilic bacterium has been shown to recognize and repair an expanded genetic base pair. Native Thermus aquaticus DNA polymerase will remove a mispaired natural base and replace it with a non-natural base to form an expanded base pair. In addition, DNA ligase will recognize a nick formed by polymerase between two non-natural base pairs and covalently attach the two strands, thus demonstrating complete repair of a bifurcated base-paired model duplex. These results add evidence to the idea that the cellular replication and repair machinery of an organism containing an expanded genetic alphabet could recognize and properly repair a site containing consecutive unnatural bases.