An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading.
An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading.
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DOI：
10.1155/2016/7363579
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发表时间：
2016
影响因子：
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通讯作者：
Alapati SB
中科院分区：
文献类型：
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作者：
Jones TD;Kefi A;Sun S;Cho M;Alapati SB
导言。HYSTEM-C™是一种商用可注射水凝胶，由聚乙二醇二丙烯酸酯(PEGDA)、透明质酸(HA)和明胶(Gn)组成。这些成分可以通过机械调节来增强细胞的活力和扩散能力。方法：研究方法。添加二硫键的聚乙二醇单甲氧基乙二胺的浓度从0.5%到8.0% (w/v)变化，以确定临床注射的最佳浓度。我们评估了人牙髓干细胞(HDPSCs)在2% (w/v)PEGSSDA-HA-Gn水凝胶中的细胞活性。HA ： Gn的体积比从100 ： 0到25 ： 75不同，以促进hDPSC的扩散。在我们的模型中加入了纤维连接蛋白(FN)，以确定细胞外基质蛋白浓度对hDPSC行为的影响。结果。我们的初步数据表明，随着PEGSSDA交联剂浓度的增加，水凝胶的凝胶时间缩短。PEGSSDA-HA-Gn与人DPSCs具有良好的生物相容性，增加HA- ： -Gn的比例可使细胞存活时间延长14天。此外，在PEGDA-HA-Gn水凝胶中加入纤维粘连蛋白后，随着时间的推移，细胞增殖显著增加，当Fn浓度为0.1 μg/mL时，细胞铺展显著增加。结论。这项研究表明，基于聚乙二醇基的可注射水凝胶主要在细胞外基质(ECM)存在的情况下维持hDPSC的活性并促进细胞扩散。
Introduction. HyStem-C™ is a commercially available injectable hydrogel composed of polyethylene glycol diacrylate (PEGDA), hyaluronan (HA), and gelatin (Gn). These components can be mechanically tuned to enhance cell viability and spreading. Methods. The concentration of PEGDA with an added disulfide bond (PEGSSDA) was varied from 0.5 to 8.0% (w/v) to determine the optimal concentration for injectable clinical application. We evaluated the cell viability of human dental pulp stem cells (hDPSCs) embedded in 2% (w/v) PEGSSDA-HA-Gn hydrogels. Volume ratios of HA : Gn from 100 : 0 to 25 : 75 were varied to encourage hDPSC spreading. Fibronectin (Fn) was added to our model to determine the effect of extracellular matrix protein concentration on hDPSC behavior. Results. Our preliminary data suggests that the hydrogel gelation time decreased as the PEGSSDA cross-linker concentration increased. The PEGSSDA-HA-Gn was biocompatible with hDPSCs, and increased ratios of HA : Gn enhanced cell viability for 14 days. Additionally, cell proliferation with added fibronectin increased significantly over time at concentrations of 1.0 and 10.0 μg/mL in PEGDA-HA-Gn hydrogels, while cell spreading significantly increased at Fn concentrations of 0.1 μg/mL. Conclusions. This study demonstrates that PEG-based injectable hydrogels maintain hDPSC viability and facilitate cell spreading, mainly in the presence of extracellular matrix (ECM) proteins.