Kinetics and differentiation of somite cells forming the vertebral column: studies on human and chick embryos
Kinetics and differentiation of somite cells forming the vertebral column: studies on human and chick embryos
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形成脊柱的体细胞的动力学和分化：对人类和鸡胚胎的研究
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
B. Christ
中科院分区：
文献类型：
--
作者：
J. Wilting;H. Kurz;B. Brand;G. Steding;Yuan Yang;H. Hasselhorn;H. Epperlein;B. Christ
我们已经研究了体节细胞的动力学与增殖细胞核抗原（PCNA/细胞周期蛋白）的抗体在人类和鸡胚，并与BrdU抗BrdU的方法在鸡胚，以探讨是否可以解释不同的增殖率的发育脊柱的同色异谱模式。此外，我们应用抗体对分化标记的软骨和肌细胞的体节，以研究增殖和分化之间的相关性。人胚和鸡胚脊柱的增殖模式无主要差异。在检查的所有阶段，细胞密度是在尾侧硬节的一半比在颅侧的一半。横向上，尾侧硬结的一半，这引起了神经弓，其特点是由一个更高的增殖活性比头侧的一半。虽然存在高变异性，但标记指数显示具有两种增殖标记物的两半之间存在显著差异。随着软骨形成分化的开始，只有软骨膜细胞保持高增殖率。在胎儿发育期间，神经弓及其突起并置生长。即使在最早的阶段，几乎没有PCNA或BrdU的结蛋白阳性肌节细胞的人和鸡胚胎的免疫染色。轴向上，椎间盘原基的致密间充质中的增殖率高于椎体原基。在胎儿发育过程中，椎体和椎间盘交界处的细胞增殖，表明贴壁生长。我们的研究结果表明，局部差异的近轴中胚层的增殖率存在，并可能是一个重要的机制，建立同色异谱模式的脊柱在人和鸡胚。
We have studied the kinetics of somite cells with an antibody against proliferating cell nuclear antigen (PCNA/cyclin) in human and chick embryos, and with the BrdU anti-BrdU method in chick embryos, to investigate whether the metameric pattern of the developing vertebral column can be explained by different proliferation rates. Furthermore we applied antibodies against differentiation markers of chondrogenic and myogenic cells of the somites in order to study the correlation between proliferation and differentiation. There are no principal differences in the proliferation pattern of the vertebral column between human and chick embryos. In all stages examined, the cell density is higher in the caudal sclerotome halves than in the cranial halves. Laterally, the caudal sclerotome halves, which give rise to the neural arches, are characterized by a higher proliferative activity than the cranial halves. Although there is a high variability, the labelling indices show significant differences between the two halves with both proliferation markers. With the onset of chondrogenic differentiation, only the perichondrial cells retain a high proliferation rate. During fetal development, the neural arches and their processes grow appositionally. Even at the earliest stages, there is practically no immunostaining for PCNA or BrdU in the desmin-positive myotome cells of human and chick embryos. Axially, a higher proliferation rate is found in the condensed mesenchyme of the anlagen of the intervertebral discs than in the anlagen of the vertebral bodies. During fetal development, cells at the borders between vertebral bodies and intervertebral discs proliferate, indicating appositional growth. Our results show that local differences in the proliferation rates of the paraxial mesoderm exist, and may be an important mechanism for the establishment of the metameric pattern of the vertebral column in human and chick embryos.
T-钙粘蛋白的表达与鸟类胚胎躯干中神经嵴细胞的迁移交替出现。
DOI：
10.1242/dev.111.1.15
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Ranscht,B;Bronner-Fraser,M
通讯作者：
Bronner-Fraser,M
DOI：
10.1073/pnas.87.15.5672
发表时间：
1990-08-01
影响因子：
11.1
作者：
LEE, SH;HURWITZ, J
通讯作者：
HURWITZ, J