Proteomic analysis of the transition from quiescent to proliferating stages in rat liver hepatectomy model
Proteomic analysis of the transition from quiescent to proliferating stages in rat liver hepatectomy model
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DOI：
10.1002/pmic.200500322
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发表时间：
2006-05-01
期刊：
影响因子：
3.4
通讯作者：
He, Dacheng
中科院分区：
文献类型：
--
作者：
Guo, Fuzheng;Nian, Hui;He, Dacheng
70%（或2/3）部分肝切除（PHx）大鼠肝脏模型为研究肝细胞从静止期到增殖期的转变和调节提供了有效的介质。尽管基因表达模式已受到严格审查，但差异蛋白质组学研究可能有助于揭示该过程启动和调节的机制。采用二维凝胶电泳分析两组70％PHx后7小时和假手术对照组的蛋白质组变化，凝胶上分别有907±33和910±64个斑点。使用ImageMaster软件识别出12个下调点和26个上调点，并通过基质辅助激光解吸/电离质谱-四极杆飞行时间质谱和/或串联质谱再确认来鉴定。一些差异蛋白与应激防御、脂质代谢和大分子生物合成有关，而另一些则被证明参与调节与肝再生相关的转录因子。根据我们的数据和相关科学文献，提出了肝再生的“蛋白质组学模型”，以解释反映细胞从静止状态向增殖状态转变的差异蛋白质组模式，包括但不限于70% PHx后的大鼠肝脏。
The 70% (or 2/3) partial hepatectomy (PHx) rat liver model provides an effective medium for study of the transition and regulation of hepatocytes from quiescent to proliferating phase. Although the gene expression pattern has come under intense scrutiny, a differential proteomic study could help to reveal the mechanism of how the process is initiated and regulated. The proteomic changes were analyzed in two groups, 7 h after 70% PHx test group and sham-operation control group, by two-dimensional gel electrophoresis with 907 +/- 33 and 910 +/- 64 spots on gels, respectively. Twelve down-regulated spots and twenty-six up-regulated spots were recognized using ImageMaster software and were identified by matrix-assisted laser desorption/ionization-mass spectrometry-quadrupole time of flight mass spectrometry and/or tandem mass spectrometry reconfirmation. Some of the differential proteins were associated with stress defense, lipid metabolism, and macromolecular biosynthesis while the others were shown to be involved in regulating transcript factors associated with liver regeneration. A "proteomic model" for liver regeneration was suggested based on our data and related scientific literature to interpret the differential proteome pattern that reflected the transition of cells from quiescent to proliferating state, including but not limited to the rat liver after 70% PHx.