Synthesis and cytotoxicity assessment of superparamagnetic iron-gold core-shell nanoparticles coated with polyglycerol
Synthesis and cytotoxicity assessment of superparamagnetic iron-gold core-shell nanoparticles coated with polyglycerol
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DOI：
10.1016/j.jcis.2010.01.038
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发表时间：
2010-05-01
影响因子：
9.9
通讯作者：
Khakpash, N.
中科院分区：
文献类型：
--
作者：
Jafari, T.;Simchi, A.;Khakpash, N.
采用反胶束法制备了核壳型铁金(Fe@Au)纳米粒子，研究了水与表面活性剂的摩尔比(W)对纳米粒子的尺寸、粒径分布和磁性的影响。采用四甲基偶氮唑盐比色法检测纳米粒的细胞毒性。为了使颗粒功能化以用于MRI成像和靶向给药，通过硫醇封端和缩水甘油聚合的方法在颗粒上包覆了聚甘油。用X射线衍射仪、透射电子显微镜、振动样品磁强计、紫外-可见光谱、傅里叶变换红外光谱等手段对样品进行了表征。结果表明，随着水与表面活性剂摩尔比(W)的增大，纳米粒子的尺寸和尺寸分布增大。这些颗粒是球形的，上面有一层薄薄的金子。观察到铁核上的金壳呈互补生长。同时，观察到磁珠和磁性胶体纳米晶团簇两种类型的团聚现象，这取决于w值。磁性测量研究揭示了纳米颗粒的超顺磁性行为。四甲基偶氮唑盐实验结果表明，合成的纳米颗粒无毒，有望应用于生物医学领域。(C)2010 Elsevier Inc.保留所有权利。
Core-shell iron-gold (Fe@Au) nanoparticles were synthesized by a facile reverse micelle procedure and the effect of water to surfactant molar ratio (w) on the size, size distribution and magnetic properties of the narroparticles was studied. MTT assay was utilized to evaluate the cell toxicity of the nanoparticles. To functionalize the particles for MRI imaging and targeted drug delivery, the particles were coated by polyglycerol through capping with thiol followed by polymerization of glycidol. The characteristics of the particles were examined by X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometere (VSM), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FT-IR). It was found that the size and size distribution of the nanoparticles increase by increasing the water to surfactant molar ratio (w). The particles were spherical in shape with a thin layer of gold. Complementary growth of the gold shell on the iron core was noticed. Meanwhile, two types of agglomeration including magnetic beads and magnetic colloidal nanocrystals clusters were observed dependent on the w-value. The magnetic measurement studies revealed the superparamagnetic behavior of the nanoparticles. MTT assay result indicated the synthesized nanoparticles are nontoxic that will be useful for biomedical applications. (C) 2010 Elsevier Inc. All rights reserved.