PhagoSight: an open-source MATLAB® package for the analysis of fluorescent neutrophil and macrophage migration in a zebrafish model.
PhagoSight: an open-source MATLAB® package for the analysis of fluorescent neutrophil and macrophage migration in a zebrafish model.
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Phagosight：用于分析斑马鱼模型中荧光中性粒细胞和巨噬细胞迁移的开源MATLAB®包装。
DOI：
10.1371/journal.pone.0072636
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Reyes-Aldasoro CC
中科院分区：
文献类型：
--
作者：
Henry KM;Pase L;Ramos-Lopez CF;Lieschke GJ;Renshaw SA;Reyes-Aldasoro CC
斑马鱼幼鱼中的中性粒细胞迁移越来越多地被用作研究这些白细胞对细胞炎症反应的不同决定因素的反应的模型。然而，它仍然具有挑战性的提取全面的信息描述的行为的中性粒细胞从多维数据集获得的宽视场或共聚焦显微镜。在这里，我们描述了PhagoSight，一个开源软件包的分割，跟踪和可视化的三维迁移吞噬细胞。PhagoSight中的算法提取了大量总结中性粒细胞行为的测量值，但这些测量值可能适用于任何移动的荧光细胞。为了获得一组有用的变量，量化中性粒细胞迁移行为的各个方面，并证明PhagoSight的效用，我们评估了迁移中性粒细胞的体积变化。当中性粒细胞向受伤的斑马鱼的伤口区域迁移时，细胞体积增加。PhagoSight在GNU通用公共许可证下以MATLAB® m-files的形式公开提供。合成数据集和全面的用户手册可从http://www.phagosight.org获得。
Neutrophil migration in zebrafish larvae is increasingly used as a model to study the response of these leukocytes to different determinants of the cellular inflammatory response. However, it remains challenging to extract comprehensive information describing the behaviour of neutrophils from the multi-dimensional data sets acquired with widefield or confocal microscopes. Here, we describe PhagoSight, an open-source software package for the segmentation, tracking and visualisation of migrating phagocytes in three dimensions. The algorithms in PhagoSight extract a large number of measurements that summarise the behaviour of neutrophils, but that could potentially be applied to any moving fluorescent cells. To derive a useful panel of variables quantifying aspects of neutrophil migratory behaviour, and to demonstrate the utility of PhagoSight, we evaluated changes in the volume of migrating neutrophils. Cell volume increased as neutrophils migrated towards the wound region of injured zebrafish. PhagoSight is openly available as MATLAB® m-files under the GNU General Public License. Synthetic data sets and a comprehensive user manual are available from http://www.phagosight.org.
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影响因子：
3.9
作者：
Hellriegel, Christian;Gratton, Enrico
通讯作者：
Gratton, Enrico
DOI：
10.1109/tcom.1967.1089532
发表时间：
1967-01-01
期刊：
IEEE TRANSACTIONS ON COMMUNICATION TECHNOLOGY
影响因子：
--
作者：
KAILATH, T
通讯作者：
KAILATH, T
影响因子：
10.6
作者：
Grau, V;Mewes, AUJ;Warfield, SK
通讯作者：
Warfield, SK
影响因子：
2.7
作者：
Collins, Tony J.
通讯作者：
Collins, Tony J.
影响因子：
5.5
作者：
Karlsson, Thommie;Glogauer, Michael;Magalhaes, Marco A. O.
通讯作者：
Magalhaes, Marco A. O.