Chronic fructose intake accelerates non-alcoholic fatty liver disease in the presence of essential hypertension
Chronic fructose intake accelerates non-alcoholic fatty liver disease in the presence of essential hypertension
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DOI：
10.1016/j.jdiacomp.2015.10.008
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发表时间：
2016-01-01
影响因子：
3
通讯作者：
Baldo, Marcelo Perim
中科院分区：
文献类型：
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作者：
Lirio, Layla Mendonca;Forechi, Ludimila;Baldo, Marcelo Perim
背景：代谢综合征的日益流行与食品工业中果糖的使用增加有关。事实上，在苏打水和果汁等加糖饮料中，果糖作为成分的使用有所增加。因此，我们假设高血压大鼠摄入果糖会导致代谢紊乱和非酒精性脂肪肝的预后较差。方法：6周龄的雄性Wistar和SHR大鼠给予水或果糖（10%）6周。每两周测量一次血糖，并在随访结束时评估胰岛素和葡萄糖敏感性测试。通过体积描记法测量收缩压。收集瘦体重和腹部脂肪量并称重。对肝脏组织进行分析以确定间质脂肪沉积和纤维化。结果：摄入高果糖的动物的空腹血糖升高，与血压水平无关。此外，在正常血压大鼠和主要是高血压大鼠摄入果糖后观察到胰岛素抵抗。果糖摄入导致两组的甘油三酯水平增加 2.5 倍。果糖摄入量不会改变瘦体重。然而，我们发现，果糖摄入量显着增加血压正常大鼠的腹部脂肪沉积，但高血压大鼠则不然。然而，长期摄入果糖只会增加高血压大鼠肝脏中的脂肪沉积和纤维化。结论：我们证明，在正常血压和高血压大鼠中，果糖摄入会增加甘油三酯和腹部脂肪沉积，并导致胰岛素抵抗。然而，摄入果糖的高血压大鼠也会出现间质脂肪沉积和肝脏纤维化。 (C) 2016 Elsevier Inc. 保留所有权利。
Background: The growing epidemic of metabolic syndrome has been related to the increased use of fructose by the food industry. In fact, the use of fructose as an ingredient has increased in sweetened beverages, such as sodas and juices. We thus hypothesized that fructose intake by hypertensive rats would have a worse prognosis in developing metabolic disorder and non-alcoholic fatty liver disease.Methods: Male Wistar and SHR rats aged 6 weeks were given water or fructose (10%) for 6 weeks. Blood glucose was measured every two weeks, and insulin and glucose sensitivity tests were assessed at the end of the follow-up. Systolic blood pressure was measure by plethysmography. Lean mass and abdominal fat mass were collected and weighed. Liver tissue was analyzed to determine interstitial fat deposition and fibrosis.Results: Fasting glucose increased in animals that underwent a high fructose intake, independent of blood pressure levels. Also, insulin resistance was observed in normotensive and mostly in hypertensive rats after fructose intake. Fructose intake caused a 2.5-fold increase in triglycerides levels in both groups. Fructose intake did not change lean mass. However, we found that fructose intake significantly increased abdominal fat mass deposition in normotensive but not in hypertensive rats. Nevertheless, chronic fructose intake only increased fat deposition and fibrosis in the liver in hypertensive rats.Conclusions: We demonstrated that, in normotensive and hypertensive rats, fructose intake increased triglycerides and abdominal fat deposition, and caused insulin resistance. However, hypertensive rats that underwent fructose intake also developed interstitial fat deposition and fibrosis in liver. (C) 2016 Elsevier Inc. All rights reserved.