Distribution and Variation of Serotypes and Pneumococcal Surface Protein A Clades of Streptococcus pneumoniae Strains Isolated From Adult Patients With Invasive Pneumococcal Disease in Japan.
Distribution and Variation of Serotypes and Pneumococcal Surface Protein A Clades of Streptococcus pneumoniae Strains Isolated From Adult Patients With Invasive Pneumococcal Disease in Japan.
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DOI：
10.3389/fcimb.2021.617573
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发表时间：
2021
影响因子：
5.7
通讯作者：
Oishi K
中科院分区：
文献类型：
--
作者：
Chang B;Kinjo Y;Morita M;Tamura K;Watanabe H;Tanabe Y;Kuronuma K;Fujita J;Oshima K;Maruyama T;Abe S;Kasahara K;Nishi J;Kubota T;Ohnishi M;Suga S;Oishi K
肺炎球菌表面蛋白 A (PspA) 是肺炎链球菌的一种表面蛋白，可能是新型肺炎球菌疫苗的候选抗原。本研究调查了日本成人侵袭性肺炎球菌病 (IPD) 致病菌株 PspA 进化枝的分布。 2014-2019年从成人IPD病例中分离出的1,939株菌株中，确定了1,932株（99.6%）的PspA进化枝，其余7株（0.4%）未检测到pspA。 PspA 进化枝 1-6 分别在 786 (40.5%)、291 (15.0%)、443 (22.8%)、369 (19.0%)、33 (1.7%) 和 6 (0.3%) 菌株中检测到。在两种不可分型和两种血清型 35B 肺炎球菌中鉴定出新的 PspA 进化枝 (0.2%)。进化枝1和进化枝2的比例分别呈现显着下降和上升的趋势。此外，肺炎球菌菌株的 PspA 进化枝部分依赖于血清型和序列类型。 13价肺炎球菌结合疫苗（PCV13）和23价肺炎球菌多糖疫苗（PPSV23）所含血清型的大多数菌株属于PspA进化枝1或3。相反，非疫苗血清型中的进化枝分布比疫苗血清型肺炎球菌的分布更广泛。我们的研究结果表明，几乎所有成人 IPD 肺炎球菌菌株都表达 PspA 进化枝 1-4，尤其是非疫苗血清型。这些结果可能有助于开发新型 PspA 肺炎球菌疫苗。
Pneumococcal surface protein A (PspA) is a surface protein of Streptococcus pneumoniae that may be a candidate antigen for new pneumococcal vaccines. This study investigates the distribution of PspA clades of the causative strains of adult invasive pneumococcal disease (IPD) in Japan. Of the 1,939 strains isolated from cases of adult IPD during 2014–2019, the PspA clades of 1,932 (99.6%) strains were determined, and no pspA was detected in the remaining 7 strains (0.4%). PspA clades 1–6 were detected in 786 (40.5%), 291 (15.0%), 443 (22.8%), 369 (19.0%), 33 (1.7%), and 6 (0.3%) strains, respectively. New PspA clades (0.2%) were identified in two non-typeable and two serotype 35B pneumococci. The proportions of clade 1 and clade 2 showed significantly decreased and increased trends, respectively. Furthermore, the PspA clade of pneumococcal strains was partially serotype- and sequence type-dependent. The majority of strains belonging to serotypes contained in both the 13-valent pneumococcal conjugate vaccine (PCV13) and the 23-valent pneumococcal polysaccharide vaccine (PPSV23) belonged to PspA clades 1 or 3. In contrast, the distribution of clades in non-vaccine serotypes was wider than that of vaccine serotype pneumococci. Our findings demonstrate that almost all pneumococcal strains from adult IPD express PspA clades 1–4, especially for non-vaccine serotypes. These results may be useful for the development of a new pneumococcal vaccine with PspA.