GC-MS study of compounds isolated from Coffea arabica flowers by different extraction techniques
GC-MS study of compounds isolated from Coffea arabica flowers by different extraction techniques
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DOI：
10.1002/jssc.201300458
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发表时间：
2013-09-01
影响因子：
3.1
通讯作者：
Camilo Duran, Diego
中科院分区：
文献类型：
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作者：
Stashenko, Elena E.;Rene Martinez, Jairo;Camilo Duran, Diego
采用顶空法、萃取法和蒸馏法从鲜咖啡花中分离挥发性和半挥发性化合物。静态HS固相微萃取（HS-SPME），微波辅助HS-SPME（MW-HS-SPME）与同时水蒸馏，和萃取与己烷或超临界CO2分离的混合物中，约150种不同的化学物质被确定或暂时确定的GC-MS分析。正十五烷（20-37%相对峰面积，RPA）是来自鲜花的HS组分中最丰富的化合物，其次是8-十七烯（8-20% RPA）和香叶醇（6-14% RPA）。烃类（主要是C-13-C-30石蜡）是所有吸附萃取（HS-SPME，MW-HS-SPME，馏出物）中的主要化合物类别，其次是萜类化合物或含氧化合物（随分离技术而变化）。咖啡因是咖啡果实和咖啡豆的一种独特成分，在C.阿拉比卡花。
Headspace (HS), extractive, and distillative methods were employed to isolate volatile and semivolatile compounds from fresh Coffea arabica flowers. Static HS solid-phase microextraction (HS-SPME), microwave-assisted HS-SPME (MW-HS-SPME) with simultaneous hydrodistillation, and extraction with hexane or supercritical CO2-isolated mixtures in which around 150 different chemical substances were identified or tentatively identified by GC-MS analysis. n-Pentadecane (20-37% relative peak area, RPA) was the most abundant compound in the HS fractions from fresh flowers, followed by 8-heptadecene (8-20% RPA) and geraniol (6-14% RPA). Hydrocarbons (mostly C-13-C-30 paraffins) were the predominant compound class in all the sorptive extractions (HS-SPME, MW-HS-SPME, distillate), followed by terpenoids or oxygenated compounds (which varied with the isolation technique). Caffeine, a distinctive component of coffee fruits and beans, was also found in relatively high amounts in the supercritical CO2 extract of C. arabica flowers.