Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable antigenhi and exhibit unique signaling characteristics.
Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable antigenhi and exhibit unique signaling characteristics.
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DOI：
10.4049/jimmunol.149.8.2533
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发表时间：
1992-10
影响因子：
4.4
通讯作者：
D. Allman;S. Ferguson;M. Cancro
中科院分区：
文献类型：
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作者：
D. Allman;S. Ferguson;M. Cancro
成人外周血新近产生的B细胞是否与新生儿脾脏中未成熟的B细胞在功能上等同尚不清楚。我们已经确定了成人脾B细胞亚群，其表型和体外特征与新生儿B细胞非常相似。这些细胞由细胞表面表型热稳定Aghi（HSAhi）定义，并且构成成人脾B细胞池的10至15%。成人中的HSAhi B细胞具有未成熟表型B220 lo sIgMhi，并且是50% sIgD+。此外，在亚致死量照射后，在自我重建的成人中新产生的脾B细胞的初始波表达类似的表型。与之前关于新生儿B细胞的数据一致，来自正常或自我重建小鼠的HSAhi细胞对抗IgM抗体的刺激不敏感，但在LPS刺激后会增殖，并且如果给予适当的T细胞帮助，则会产生初级抗体反应。与新生细胞相反，HSAhi成人B细胞对PMA加离子霉素的刺激是难治的。总之，这些数据表明，成人外周HSAhi B细胞对应于最近产生的B细胞，其信号传导特征与先前描述的B细胞亚群不同。
Whether recently generated peripheral B cells in adults are functionally equivalent to immature B cells in the neonatal spleen is unknown. We have identified a splenic B cell subpopulation in adults whose phenotypic and in vitro characteristics closely resemble those of neonatal B cells. These cells are defined by the cell surface phenotype heat-stable Aghi (HSAhi), and make up 10 to 15% of the adult splenic B cell pool. HSAhi B cells in adults bear the immature phenotype B220lo sIgMhi, and are 50% sIgD+. Furthermore, after sublethal irradiation, the initial wave of newly generated splenic B cells in self-reconstituting adults express a similar phenotype. In keeping with previous data on neonatal B cells, HSAhi cells from either normal or self-reconstituting mice are refractory to stimulation with anti-IgM antibodies, yet proliferate upon LPS stimulation, and generate primary antibody responses if given appropriate T cell help. In contrast to neonatal cells, HSAhi adult B cells are refractory to stimulation with PMA plus ionomycin. Together, these data suggest that peripheral HSAhi B cells in adults correspond to recently generated B cells, whose signaling characteristics are distinct from previously described B cell subsets.