EVALUATION OF THE HYBRID CAPTURE HUMAN PAPILLOMAVIRUS DEOXYRIBONUCLEIC-ACID DETECTION TEST
EVALUATION OF THE HYBRID CAPTURE HUMAN PAPILLOMAVIRUS DEOXYRIBONUCLEIC-ACID DETECTION TEST
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DOI：
10.1016/0002-9378(95)90629-0
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发表时间：
1995-11-01
影响因子：
9.8
通讯作者：
WRIGHT, TC
中科院分区：
文献类型：
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作者：
SUN, XW;FERENCZY, A;WRIGHT, TC
目的：我们的目的是评估一种新的研究设计的灵敏度和准确性：用杂交捕获试验和聚合酶链反应检测520名妇女的宫颈标本中的人乳头瘤病毒脱氧核糖核酸。在254例无宫颈上皮内瘤变证据的妇女样本中，106例（42%）和211例（21%）样本中用杂交捕获法检测到人乳头瘤病毒脱氧核糖核酸。79%）。杂交捕获与聚合酶链反应之间存在良好的相关性。杂交捕获正确地识别了92%的样本发现含有高或中等致癌风险的人乳头瘤病毒类型的聚合酶链反应。虽然杂交捕获可以量化的人乳头瘤病毒脱氧核糖核酸的量存在于一个样本中，没有观察到与杂交捕获检测到的人乳头瘤病毒脱氧核糖核酸的相对量和宫颈病变的等级之间的相关性。结论：杂交捕获测试是一种敏感和准确的方法，用于识别人乳头瘤病毒类型的高和中等致癌风险的临床标本。
OBJECTIVE: Our purpose was to evaluate the sensitivity and accuracy of a new,STUDY DESIGN: Cervical samples from 520 women were assayed for human papillomavirus deoxyribonucleic add with both the Hybrid Capture test and polymerase chain reaction.RESULTS: Human papillomavirus deoxyribonucleic acid was detected with Hybrid Capture in 106 (42%) of 254 samples from women with no evidence of cervical intraepithelial neoplasia and 211 (79%) of 266 with cervical intraepithelial lesions or cervical cancer. There was a good correlation between Hybrid Capture and polymerase chain reaction. Hybrid Capture correctly identified 92% of samples found to contain a human papillomavirus type with a high or intermediate oncogenic risk with polymerase chain reaction. Although Hybrid Capture can quantify the amount of human papillomavirus deoxyribonucleic acid present in a sample, no correlation was observed between the relative amount of human papillomavirus deoxyribonucleic acid detected with Hybrid Capture and the grade of cervical lesion.CONCLUSION: The Hybrid Capture test is a sensitive and accurate method for identifying human papillomavirus types of high and intermediate oncogenic risk in clinical specimens.