Production of odd chain polyunsaturated fatty acids byMortierella fungi
Production of odd chain polyunsaturated fatty acids byMortierella fungi
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被孢霉属真菌生产奇链多不饱和脂肪酸
DOI：
10.1007/bf02657620
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发表时间：
1991
影响因子：
2
通讯作者：
H. Yamada
中科院分区：
文献类型：
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作者：
S. Shimizu;H. Kawashima;K. Akimoto;Y. Shinmen;H. Yamada
在花生四烯酸产生菌中，高山被孢霉1 S-4菌株具有较高的奇数链多不饱和脂肪酸（PUFA）产量。该菌主要积累5，8，11，M-顺式十九碳四烯酸。在5%正十七烷和1%酵母膏的培养基中，C19：4：4酸的积累量达到44.4mg/g干菌丝（0.68mg/mL培养液）。该值占总脂肪酸的11.2%，其中奇数链脂肪酸占总脂肪酸的95%以上。A5去饱和的特异性抑制剂芝麻素的加入引起C19：3酸的增加和伴随的C19：4酸的减少。另一方面，不能产生C-20多不饱和脂肪酸的被孢霉属物种在与具有奇数链骨架的脂肪基质生长时积累C-17酸，但不积累C-19多不饱和脂肪酸。奇数链多不饱和脂肪酸分布在中性和极性脂质中。推测C19：4酸的生物合成途径模拟花生四烯酸的n-6途径，如下：C17：0→ C17：1→ C17：2→ C17：3→ C19：3→ C19：4酸。
A soil isolate,Mortierella alpina1S-4, was found to show high production of odd chain polyunsaturated fatty acids (PUFAs) among various arachidonic acid-producingMortierellastrains tested. The fungus mainly accumulated 5,8,11,M-cis-nonadecatetraenoic acid. With 5%n-hepta-decane and 1% yeast extract as growth substrates, the amount of C19:4:4 acid accumulated reached 44.4 mg/g dry mycelia (0.68 mg/mL of culture broth). This value accounted for 11.2% of the total fatty acids in the extracted lipids from mycelia, and odd chain fatty acids comprised over 95% of the total mycelial fatty acids. The addition of sesamin, a specific inhibitor of A5 desaturation, caused an increase in C19:3acid and an accompanying decrease in C19:4acid. On the other hand, species ofMortierellathat could not produce C-20 PUFAs accumulated C-17 acids, but no C-19 PUFAs, when grown with fatty substrates with an odd chain skeleton. The odd chain PUFAs were distributed in both neutral and polar lipids. The biosynthetic route to C19:4acid was presumed to mimic the n-6 route to arachidonic acid as follows: C17:0→ C17:1→ C17:2→ C17:3→ C19:3→ C19:4acids.
Minami，Kohtaro：农业生物化学。
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