Making and working with hydrogen sulfide The chemistry and generation of hydrogen sulfide in vitro and its measurement in vivo: A review
Making and working with hydrogen sulfide The chemistry and generation of hydrogen sulfide in vitro and its measurement in vivo: A review
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DOI：
10.1016/j.freeradbiomed.2009.09.018
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发表时间：
2009-11-15
影响因子：
7.4
通讯作者：
Moore, Kevin P.
中科院分区：
文献类型：
--
作者：
Hughes, Martin N.;Centelles, Miguel N.;Moore, Kevin P.
硫化氢正迅速成为一种重要的血管活性物质，在健康和疾病中形成。它的生物学作用集中在它与血红素蛋白的反应和激活K-ATP通道的能力上。硫化氢是炎症和神经系统的信号分子，特别是心血管系统，在那里它调节血管张力、心脏做功和发挥心脏保护作用。这导致了大量的论文，其中使用体外产生的硫化氢的作用来刺激生物反应，并使用各种方法来测量生物液中这种化合物的浓度。了解用于测量硫化氢浓度的分析技术中的化学和固有问题，对于我们扩大对硫化氢生物学的知识至关重要。在这篇简短的综述中，我们将讨论硫化氢的化学，包括硫化氢的来源，在生理pH下的形态，硫化物对好氧氧化的敏感性，以及用于测量溶液中硫化氢浓度的方法，包括生物液体。我们还简要概述了基因敲除动物和体内参与硫化氢形成的酶的抑制。(C)2009 Elsevier Inc.保留所有权利。
Hydrogen sulfide is rapidly emerging as an important vasoactive mediator formed in health and disease. Its biological action is centered on its reactivity with heme-proteins and its ability to activate K-ATP channels. Hydrogen sulfide is a signalling molecule of the inflammatory and nervous systems, and in particular the cardiovascular system where it regulates vascular tone, cardiac work, and exerts cardioprotection.This has led to an explosion of papers in which the role of hydrogen sulfide generated in vitro has been used to stimulate biological responses, and where a variety of methods have been used to measure the concentration of this compound in biological fluids. Understanding the chemistry and the inherent problems in the analytical techniques used to measure hydrogen sulfide concentrations is critical to our expanding knowledge on the biology of hydrogen sulfide. In this brief review we will cover the chemistry of hydrogen sulfide, including sources of hydrogen sulfide, its speciation at physiological pH, the susceptibility of sulfide to aerobic oxidation, and the methods used to measure hydrogen sulfide concentrations in solution, including biological fluids. We also give a brief overview of knockout animals and inhibition of the enzymes involved in the formation of hydrogen Sulfide in vivo. (C) 2009 Elsevier Inc. All rights reserved.